COMRADES 可用于体内 RNA 结构和相互作用的测定。
COMRADES determines in vivo RNA structures and interactions.
机构信息
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
Department of Genetics, University of Cambridge, Cambridge, UK.
出版信息
Nat Methods. 2018 Oct;15(10):785-788. doi: 10.1038/s41592-018-0121-0. Epub 2018 Sep 10.
The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.
RNA 的结构灵活性是基础生物过程的基础，但目前还没有方法可以探索 RNA 在体内采用的多种构象。我们开发了匹配 RNA 的交联和深度测序（COMRADES）方法，用于深入捕获 RNA 构象，并构建了 RNA 结构集合检索的管道。我们使用 COMRADES 确定了寨卡病毒 RNA 基因组在细胞内的结构，并鉴定了与人类非编码 RNA 的多个特定位置相互作用。
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