El-Badry Ayman A, Hamdy Doaa A, Abd El Wahab Wegdan M
Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Medical Parasitology, College of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Parasitol Res. 2018 Dec;117(12):3775-3780. doi: 10.1007/s00436-018-6078-1. Epub 2018 Sep 10.
Strongyloides stercoralis (S. stercoralis) is a soil-borne helminth but its prevalence in water samples is underestimated. The aim of the present study is to propose non-nutrient agar (NNA) as a novel culture method in the diagnosis of Strongyloides and to detect the prevalence rate of S. stercoralis larvae in tap water samples in Beni-Suef Governorate, Egypt. Eighty tap water samples were collected from Beni-Suef Governorate and each sample was filtered in three steps using three filters: one filter for microscopic examination of wet mount, while the remaining two filters were cultured on both NNA and nutrient agar plate (APC). S. stercoralis larvae, adults, and eggs were detected in two (2.5%) and four (5%) out of 80 samples after examination of wet mount preparation and cultures on both NNA and APC, respectively. NNA succeeded to maintain Strongyloides alive for up to 1 month. Our study is the first to detect Strongyloides larvae in tap water in Egypt and the first to use NNA as a novel culture media for Strongyloides.
粪类圆线虫是一种土源性蠕虫，但其在水样中的流行率被低估。本研究的目的是提出用非营养琼脂（NNA）作为诊断粪类圆线虫的一种新型培养方法，并检测埃及贝尼苏韦夫省自来水中粪类圆线虫幼虫的流行率。从贝尼苏韦夫省采集了80份自来水样本，每个样本分三步使用三个滤器进行过滤：一个滤器用于湿片镜检，其余两个滤器分别接种于NNA和营养琼脂平板（APC）上进行培养。在对湿片制备物以及NNA和APC上的培养物进行检查后，在80份样本中分别有2份（2.5%）和4份（5%）检测到粪类圆线虫幼虫、成虫和虫卵。NNA成功使粪类圆线虫存活长达1个月。我们的研究首次在埃及自来水中检测到粪类圆线虫幼虫，也是首次将NNA用作粪类圆线虫的新型培养基。
