Poonsuk Korakrit, Giménez-Lirola Luis G, Magtoto Ronaldo L, Ji Ju, Baum David H, Rademacher Christopher J, Brown Justin T, Zhang Jianqiang, Wang Chong, Main Rodger G, Zimmerman Jeffrey J
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Poonsuk, Giménez-Lirola, Magtoto, Baum, Rademacher, Brown, Zhang, Main, Zimmerman), Iowa State University, Ames, IA.
Department of Statistics, College of Liberal Arts and Sciences (Ji, Wang), Iowa State University, Ames, IA.
J Vet Diagn Invest. 2018 Nov;30(6):937-941. doi: 10.1177/1040638718798672. Epub 2018 Sep 11.
Routine testing of breeding herd oral fluid (OF) samples for porcine epidemic diarrhea virus (PEDV) IgG and/or IgA is used to track levels of PEDV immunity over time. However, OFs contain particles of feed, feces, and inorganic material that detract from the quality of the sample. We clarified swine OF samples using lyophilized chitosan-based formulas (A-C) tested by PEDV IgG and IgA ELISAs. To evaluate both the immediate and residual effects of treatment on antibody detection, samples were tested immediately post-treatment, then stored at 4°C and retested at 2, 4, and 6 days post-treatment (DPT). Formulations were shown to effectively clarify samples. Statistical analysis comparing treated to untreated OF samples at 0 DPT found that neither chitosan nor Tween 20 affected the OF ELISA IgA and IgG sample-to-positive (S/P) ratio results ( p > 0.05). Furthermore, pairwise comparisons of 0 DPT to 2, 4, and 6 DPT results detected no significant differences ( p > 0.05) in IgA and IgG S/P ratios (i.e., treated OF samples were stable over time). Therefore, chitosan efficiently clarified OF specimens without affecting the results of the PEDV IgG and IgA antibody ELISAs.
对种猪群口腔液体(OF)样本进行猪流行性腹泻病毒(PEDV)IgG和/或IgA的常规检测,用于长期追踪PEDV免疫水平。然而,口腔液体中含有饲料颗粒、粪便颗粒和无机物质,会影响样本质量。我们使用基于壳聚糖的冻干配方(A - C)对猪口腔液体样本进行澄清,并通过PEDV IgG和IgA酶联免疫吸附测定(ELISA)进行检测。为了评估处理对抗体检测的即时和残留影响,样本在处理后立即进行检测,然后在4°C下储存,并在处理后2、4和6天(DPT)重新检测。结果表明,这些配方能有效澄清样本。在0 DPT时,对处理过的和未处理的口腔液体样本进行统计分析发现,壳聚糖和吐温20均未影响口腔液体ELISA IgA和IgG样本与阳性(S/P)比值结果(p > 0.05)。此外,0 DPT与2、4和6 DPT结果的成对比较未发现IgA和IgG S/P比值有显著差异(p > 0.05)(即处理过的口腔液体样本随时间保持稳定)。因此,壳聚糖能有效澄清口腔液体样本,且不影响PEDV IgG和IgA抗体ELISA的结果。