Sarmento Luciana V, Poonsuk Korakrit, Tian Liying, Mora-Díaz Juan C, Main Rodger G, Baum David H, Zimmerman Jeffrey J, Giménez-Lirola Luis G
Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA.
J Vet Diagn Invest. 2020 Mar;32(2):324-328. doi: 10.1177/1040638720903346. Epub 2020 Feb 15.
Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We evaluated the performance of a PEDV imaging cytometry-based high-throughput neutralization test (HTNT) and compared the HTNT to a fluorescent focus neutralization (FFN) assay using serum samples from pigs of known PEDV infection status ( = 159). Estimates of diagnostic sensitivity and specificity for HTNT and FFN assays derived from receiver-operator characteristic (ROC) curve analyses showed that both PEDV FFN and HTNT provided excellent diagnostic performance. However, in the laboratory, imaging cytometry provided an objective and semi-automated approach that removed human subjectivity from the testing process and reduced the read-time of a 96-well plate to < 4 min. In addition, imaging cytometry facilitated the rapid collection and long-term storage of test images and data for further evaluation or client consultation. For PEDV and other pathogens, imaging cytometry could provide distinct advantages over classic virus neutralization or FFN assays for the detection and quantitation of neutralizing antibody.
猪流行性腹泻病毒（PEDV）是一种新出现的猪冠状病毒，给养猪业带来了巨大的经济负担。评估PEDV中和抗体水平为评估和预测群体免疫力提供了一个有价值的工具。我们评估了基于成像细胞术的PEDV高通量中和试验（HTNT）的性能，并使用已知PEDV感染状态的猪（n = 159）的血清样本，将HTNT与荧光灶中和（FFN）试验进行了比较。通过受试者工作特征（ROC）曲线分析得出的HTNT和FFN试验的诊断敏感性和特异性估计表明，PEDV FFN和HTNT均具有出色的诊断性能。然而，在实验室中，成像细胞术提供了一种客观的半自动化方法，消除了检测过程中的人为主观性，并将96孔板的读取时间缩短至<4分钟。此外，成像细胞术有助于快速收集和长期存储测试图像及数据，以供进一步评估或客户咨询。对于PEDV和其他病原体，成像细胞术在检测和定量中和抗体方面可能比经典病毒中和或FFN试验具有明显优势。
