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[将曲拉通X-100应用于青蛙嗅粘膜后嗅纤毛的再生及嗅觉电图的恢复]

[Regeneration of olfactory flagella and restoration of the electroolfactogram following application of triton X-100 to the olfactory mucosa of frogs].

作者信息

Bronshteín A A, Minor A V

出版信息

Tsitologiia. 1977;19(1):33-9.

PMID:302048
Abstract

A short-tern (1-1.5 min.) irrigation of the olfactory mucose of the frog Rana temporaria with 0.1-0.15% Triton X-100 in Ringer's solution led to the destroying of olfactory flagella but did not damage the olfactory knob and its flagellar basal bodies. Simultaneously, the generator potential of the olfactory cells-elecroolfactogram (EOG)-disappears. The olfactory cells deprived of fragella were able to produce these organelles. This process begins 2 or 3 hours following theflagellum removal, proceeds in some stages and completes within 2 or 3 days. During the flagellum regeneration the ability of olfactory cells to generate EOG is seen to resotre. The data obtained confirm the presence of receptive sites on flagellar surface.

摘要

用林格氏液中0.1 - 0.15%的曲拉通X - 100对泽蛙的嗅粘膜进行短期(1 - 1.5分钟)冲洗,会导致嗅鞭毛被破坏,但不会损伤嗅球及其鞭毛基体。同时,嗅觉细胞的发生器电位——电嗅觉图(EOG)消失。失去鞭毛的嗅觉细胞能够产生这些细胞器。这个过程在鞭毛去除后2或3小时开始,分几个阶段进行,并在2或3天内完成。在鞭毛再生过程中,嗅觉细胞产生EOG的能力得以恢复。所获得的数据证实了鞭毛表面存在感受位点。

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