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[用葡聚糖凝胶G-10柱层析法研究促胸腺淋巴细胞低分子制剂的异质性]

[Studies in heterogeneity of thymus lymphocytotropic low-molecular preparation by gel chromatography on Sephadex G-10].

作者信息

Bezvershenko I A

出版信息

Ukr Biokhim Zh. 1977 May-Jun;49(3):47-50.

PMID:302052
Abstract

The low-molecular thymus preparation obtained by dialysis and acetone precipitation is studied by gel chromatography on Sephadex G-10. Gel chromatography was performed on the column equilibrated with 6 M urea at pH 3. The column is calibrated with NADP, ATP, sucrose, glucose and KH2-PO4. The preparation was divided into two fractions with approximate molecular weights of 120-200 and 500-700 daltons.

摘要

通过透析和丙酮沉淀获得的低分子胸腺制剂，采用Sephadex G - 10凝胶色谱法进行研究。凝胶色谱在pH 3的6 M尿素平衡的柱上进行。该柱用NADP、ATP、蔗糖、葡萄糖和KH2 - PO4进行校准。该制剂被分为两个部分，分子量约为120 - 200道尔顿和500 - 700道尔顿。

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