School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, Thailand.
School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, Thailand.
J Proteomics. 2019 Feb 10;192:208-222. doi: 10.1016/j.jprot.2018.09.003. Epub 2018 Sep 8.
Dietary oils of varying fatty acid composition have been used in tilapia feeds; nevertheless, investigation of their effects on metabolism and physiological processes has been limited. Therefore, in this study, using proteomic technology, the effects of different dietary lipids (DLs) on hepatic lipid metabolism and physiological processes were investigaed in Nile tilapia (Oreochromis niloticus). Fish were fed with different DL, which included palm oil (PO), linseed oil (LO) and soybean oil (SBO) for 90 days. Growth performance appeared to be similar among the dietary group. Hepatic FA contents were reflected by DL. Dietary PO (source of saturated fatty acids; SFA) led to an increase in the hepatosomatic index as well as lipid and protein contents in liver. Dietary SBO (source of n-6 polyunsaturated fatty acids; n-6 PUFAs) increased hepatic carbohydrate contents, but decreased the hepatic protein. The proteomic analysis showed that these nutritive changes in the liver were mediated by several proteins involved in lipid, carbohydrate, and amino acid metabolism. Dietary SBO showed an increased accumulation in proteins related to oxidative stress, immune and inflammatory processes. Dietary LO (source of n-3 PUFAs) increased abundance of cytoskeleton-related proteins. qRT-PCR was performed to provide supportive information for the result of proteomic study. Similar effects of DLs on mRNA levels were observed for atp5b, krt18, and selenbp1. Combined together, dietary SFA could supply as energy reservoir for regular activites. Dietary n-3 and n-6 PUFAs led to induce vital metabolic and physiologic processes which would contribute to maintaining normal health and/or providing health-related benefits. SIGNIFICANCE: Dietary SBO increased hepatic carbohydrate contents, but decreased the hepatic protein. Different dietary lipid led to alter the abundance of several proteins (i.e., DHRS1, ATP5B, PLA2G12B, APO, AMY2A, GRP78, PRSS1, FAH, and PRSS36) involved in lipid, carbohydrate, and amino acid metabolism. Dietary SBO showed an increased accumulation in proteins (i.e., QDPR, CABC1, and PRDX6) that respond to oxidative stress, suggesting that n-6 PUFAs induce oxidative conditions. Dietary SBO led to increase the accumulation of proteins (i.e., NITR26, NCCRP1, and LCN) involved in immune and inflammatory processes. Dietary LO increased the abundant levels of cytoskeleton-related proteins (i.e., ACTB, AHNAK, ERC2, KRT18, and RILP1). Other proteins (i.e., SELENBP1, FAM46C, and ANC1) involved in other physiological processes were also modulated by DL. qRT-PCR was performed to provide supportive information of proteomic study. Similar effects of DLs on mRNA levels were observed for atp5b, krt18, and selenbp1.
不同脂肪酸组成的膳食油已用于罗非鱼饲料中；然而，其对代谢和生理过程的影响的研究仍很有限。因此，本研究采用蛋白质组学技术，研究了不同膳食脂肪（DLs）对尼罗罗非鱼肝脂质代谢和生理过程的影响。将鱼用不同的 DL 喂养 90 天，这些 DL 包括棕榈油（PO）、亚麻籽油（LO）和大豆油（SBO）。各组的生长性能似乎相似。肝脂肪酸含量反映了 DL。膳食 PO（饱和脂肪酸的来源；SFA）导致肝体比指数以及肝脂质和蛋白质含量增加。膳食 SBO（n-6 多不饱和脂肪酸的来源；n-6 PUFA）增加了肝内碳水化合物含量，但降低了肝蛋白质含量。蛋白质组学分析表明，这些肝脏营养变化是由几种参与脂质、碳水化合物和氨基酸代谢的蛋白质介导的。膳食 SBO 显示与氧化应激、免疫和炎症过程相关的蛋白质积累增加。膳食 LO（n-3 PUFA 的来源）增加了与细胞骨架相关的蛋白质的丰度。qRT-PCR 为蛋白质组学研究结果提供了支持性信息。atp5b、krt18 和 selenbp1 的 mRNA 水平也观察到了 DL 相似的影响。综合来看，膳食 SFA 可作为常规活动的能量储备。膳食 n-3 和 n-6 PUFA 导致诱导重要的代谢和生理过程，有助于维持正常健康和/或提供健康相关益处。意义：膳食 SBO 增加了肝内碳水化合物含量，但降低了肝蛋白质含量。不同的膳食脂质导致几种参与脂质、碳水化合物和氨基酸代谢的蛋白质（即 DHRS1、ATP5B、PLA2G12B、APO、AMY2A、GRP78、PRSS1、FAH 和 PRSS36）的丰度发生改变。膳食 SBO 显示与氧化应激相关的蛋白质（即 QDPR、CABC1 和 PRDX6）的积累增加，表明 n-6 PUFA 诱导氧化条件。膳食 SBO 导致与免疫和炎症过程相关的蛋白质（即 NITR26、NCCRP1 和 LCN）的积累增加。膳食 LO 增加了细胞骨架相关蛋白质（即 ACTB、AHNAK、ERC2、KRT18 和 RILP1）的丰度水平。其他参与其他生理过程的蛋白质（即 SELENBP1、FAM46C 和 ANC1）也被 DL 调节。进行 qRT-PCR 为蛋白质组学研究提供了支持性信息。atp5b、krt18 和 selenbp1 的 mRNA 水平也观察到了 DL 相似的影响。
