采用氧化镁预处理提高玉米秸秆的可发酵糖得率和浓度，用于乙醇生产。
Boosting the fermentable sugar yield and concentration of corn stover by magnesium oxide pretreatment for ethanol production.
机构信息
Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USA.
Department of Industrial and Manufacturing Systems Engineering, Kansas State University, Manhattan, KS 66506, USA.
出版信息
Bioresour Technol. 2018 Dec;269:400-407. doi: 10.1016/j.biortech.2018.08.102. Epub 2018 Sep 4.
MgO pretreatment was investigated to boost the fermentable sugars derived from corn stover with LHW pretreatment as control. Compared to LHW pretreatment, MgO pretreatment caused twice hemicellulose recovery (42 vs 21%). Double hemicellulose recovery not only didn't affect glucose yield but increased xylose yield by 13% and total sugar yield by 6% under the optimal conditions (pretreatment: 10% biomass loading, 0.1 mol/L MgO, 190 °C, and 40 min; hydrolysis: pretreated biomass loading of 10 g/100 mL, enzyme loading of 1 mL/g pretreated biomass, 50 °C, and 120 h). A total sugar concentration of 50 g/L was obtained under the above conditions. Besides, the enzyme component hydrolyzing xylan may be prone to deactivation by lignin residues in pretreated biomass. Both SEM and FTIR analyses indicate that MgO effectively disrupted the biomass structures and enlarged the exposed surface area of carbohydrates, thus boosting the enzymatic hydrolysis and fermentable sugars for ethanol production.
采用 MgO 预处理来提高玉米秸秆经 LHW 预处理后的可发酵糖得率，以 LHW 预处理作为对照。与 LHW 预处理相比，MgO 预处理使半纤维素回收率提高了一倍（42%比 21%）。在最佳条件下（预处理：生物质负荷为 10%，MgO 浓度为 0.1 mol/L，190°C，40 min；水解：预处理生物质负荷为 10 g/100 mL，酶负荷为 1 mL/g 预处理生物质，50°C，120 h），不仅不会影响葡萄糖的产量，反而会使木糖的产量增加 13%，总糖的产量增加 6%。在上述条件下可获得 50 g/L 的总糖浓度。此外，可能是由于预处理生物质中的木质素残留使水解木聚糖的酶组分容易失活。SEM 和 FTIR 分析均表明，MgO 有效地破坏了生物质结构，增加了碳水化合物的暴露表面积，从而促进了酶解和可发酵糖用于乙醇生产。
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