Sobrinho-Simões M, Stenwig A E, Nesland J M, Holm R, Johannessen J V
Pathol Res Pract. 1986 Aug;181(4):464-71. doi: 10.1016/S0344-0338(86)80086-3.
A primary mucinous carcinoma of the thyroid is reported. The tumour cells, arranged in small nests and trabecules, were surrounded by abundant intercellular substance which was positively stained for mucicarmine, alcian blue and PAS before and after diastase treatment. Diastase-resistant, PAS-positive material was also present in the tumour cells. Electron microscopy revealed neoplastic cells linked by desmosomes and not surrounded by basal lamina; the nuclei had irregular contours, prominent nucleoli and numerous pseudoinclusions; mucin granules and rough endoplasmic reticulum were the prominent organelles in the cytoplasm. Follicles and follicle-like structures were not found, either by light or by electron microscopy. A definite diagnosis could only be established after the disclosure of intense immunostaining for thyroglobulin in several groups of tumour cells. Criteria for the diagnosis are discussed and the question of histogenesis raised.
报告了一例原发性甲状腺黏液癌。肿瘤细胞呈小巢状和小梁状排列，周围有丰富的细胞间质，黏液卡红、阿尔辛蓝和淀粉酶处理前后的PAS染色均呈阳性。肿瘤细胞中也存在抗淀粉酶、PAS阳性物质。电子显微镜显示肿瘤细胞通过桥粒连接，未被基膜包围；细胞核轮廓不规则，核仁突出，有许多假包涵体；黏液颗粒和粗面内质网是细胞质中的主要细胞器。光镜和电镜均未发现滤泡和滤泡样结构。只有在几组肿瘤细胞中发现甲状腺球蛋白强烈免疫染色后才能做出明确诊断。讨论了诊断标准并提出了组织发生问题。
