Hynie S, Bojar S, Klenerová V, Wenke M
Physiol Bohemoslov. 1986;35(4):299-307.
This paper describes a simple and rapid procedure for the estimation of specific parameters (dissociation constants, Kd and maximum binding capacities, Bmax) of ligand binding to two receptor subpopulations. This procedure provides, in a few minutes, the investigator, performing the actual binding studies, the necessary information about receptor heterogeneity, enabling the investigator to plan further experiments. The procedure is based on the graphical comparison of experimental binding data (ligand binding to one or two receptor subpopulations) with the theoretical values of ligand binding to one receptor population at four levels). The values of Kd and Bmax for high- and low-affinity receptors are derived from 4 horizontal deviations of experimental data from a theoretical data plot at these levels by their comparison with tabulated deviations. The correctness of the estimated parameters can be confirmed by the comparison of experimental data with those simulated on the basis of applying the values of Kd and Bmax found in the formula for ligand binding to two receptor subpopulations. The practical applicability of this procedure was demonstrated both on simulated and experimental data, and confirmed by the well known computer programs for evaluating receptor heterogeneity, namely "LIGAND" and "Affinity spectra".
本文描述了一种简单快速的方法，用于估算配体与两个受体亚群结合的特定参数（解离常数Kd和最大结合容量Bmax）。该方法能在几分钟内为进行实际结合研究的研究者提供有关受体异质性的必要信息，使研究者能够规划进一步的实验。该方法基于将实验结合数据（配体与一个或两个受体亚群的结合）与配体在四个水平上与一个受体群体结合的理论值进行图形比较。通过将实验数据与理论数据图在这些水平上的4个水平偏差与表格偏差进行比较，得出高亲和力和低亲和力受体的Kd和Bmax值。通过将实验数据与基于应用在配体与两个受体亚群结合公式中找到的Kd和Bmax值模拟得到的数据进行比较，可以确认估算参数的正确性。该方法在模拟数据和实验数据上均展示了实际适用性，并得到了用于评估受体异质性的著名计算机程序“LIGAND”和“亲和力光谱”的证实。
