Bartosińska Joanna, Zakrzewska Ewelina, Purkot Joanna, Michalak-Stoma Anna, Kowal Małgorzata, Krasowska Dorota, Chodorowska Grażyna, Giannopoulos Krzysztof
Department of Dermatology, Venereology and Paediatric Dermatology, Medical University of Lublin, Lublin, Poland.
Experimental Hematooncology Department, Medical University of Lublin, Lublin, Poland.
Postepy Dermatol Alergol. 2018 Aug;35(4):344-350. doi: 10.5114/ada.2018.75609. Epub 2018 Aug 21.
Psoriasis with and without arthritis have common immunological mechanisms which among others involve the interactions between cytokines produced by T cells, including Th1, Th17 and Th22. Although quite a lot is known about psoriasis pathogenesis, the cause of chronic immune activation and response in the disease remains unclear. One of the negative regulators of the immune system is programmed death 1 (PD-1).
To assess the expression level of PD-1 in the peripheral T cells of psoriatic patients with and without arthritis.
The study included 23 psoriatic patients with arthritis, 52 psoriatic patients without arthritis and 52 healthy controls. The percentages of CD3+, CD4+, CD8+, CD4+PD-1+ and CD8+PD-1+ T cells were analyzed using flow cytometry.
The percentages of CD4+PD-1+ as well as CD8+PD-1+ T cells in the psoriatic patients both with and without arthritis were significantly lower than in the control group. The percentages of CD4+PD-1+ as well as CD8+PD-1+T cells were not significantly different between the psoriatic patients with and without arthritis. A significant positive correlation between PD-1 expression on the CD4+ and CD8+ T cells was found in the psoriatic patients without arthritis.
Impairment of the negative co-stimulation from PD-1 may be another common characteristic of psoriasis both with and without arthritis.
伴或不伴关节炎的银屑病具有共同的免疫机制，其中包括T细胞产生的细胞因子之间的相互作用，这些细胞因子包括Th1、Th17和Th22。尽管对银屑病发病机制已有很多了解，但该疾病中慢性免疫激活和反应的原因仍不清楚。免疫系统的负调节因子之一是程序性死亡1（PD-1）。
评估伴或不伴关节炎的银屑病患者外周血T细胞中PD-1的表达水平。
该研究纳入了23例伴关节炎的银屑病患者、52例不伴关节炎的银屑病患者和52例健康对照者。使用流式细胞术分析CD3+、CD4+、CD8+、CD4+PD-1+和CD8+PD-1+T细胞的百分比。
伴或不伴关节炎的银屑病患者中CD4+PD-1+以及CD8+PD-1+T细胞的百分比均显著低于对照组。伴关节炎和不伴关节炎的银屑病患者之间CD4+PD-1+以及CD8+PD-1+T细胞的百分比无显著差异。在不伴关节炎的银屑病患者中，发现CD4+和CD8+T细胞上的PD-1表达之间存在显著正相关。
来自PD-1的负性共刺激受损可能是伴或不伴关节炎的银屑病的另一个共同特征。
