没有证据表明GPx1、MnSOD和CAT基因的三种常见多态性对多囊卵巢综合征易感性有重大影响。

No evidence for a major effect of three common polymorphisms of the GPx1, MnSOD, and CAT genes on PCOS susceptibility.

作者信息

Salahshoor Mohammad Reza, Sohrabi Maryam, Jalili Faramarz, Jalili Parnian, Rezavand Negin, Haghnazari Lida, Jalili Cyrus

机构信息

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Kermanshah Province, Iran.

Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Kermanshah Province, Iran.

出版信息

J Cell Biochem. 2019 Feb;120(2):2362-2369. doi: 10.1002/jcb.27564. Epub 2018 Sep 11.

DOI:10.1002/jcb.27564

PMID:30206965

Abstract

AIM

Polycystic ovary syndrome (PCOS) is one of the prevalent endocrine-metabolic disorders. It is proposed that oxidative stress contributes to PCOS susceptibility and its metabolic associations. The current study aimed to investigate the influence of GPx1 (rs1050450), MnSOD (rs4880), and Catalase (rs1001179) variants with PCOS susceptibility, for the first time.

METHODS

In a case-control study, 350 Kurdish female volunteers (175 PCOS patients and 175 healthy controls) from Western Iran were studied. Genotyping for GPx1 and MnSOD were done using PCR-RFLP and for CAT the allele-specific PCR method was used.

RESULTS

The percentage of patients suffering from hirsutism, acne, and acanthosis among patients with PCOS were 44.6%, 30.3%, and 14.9%, respectively. Distribution of alleles among patients suffering from PCOS versus healthy women was 'Pro' (69.1% vs 68.8%) and 'Leu' (31.4% vs 31.2%) for Gpx1, 'Ala' (61.43% vs 56.57%) and 'Val' (38.57% vs 43.43%) for MnSOD, and 'C' (83.43% vs 84.57%) and 'T' (16.57% vs 15.43%) for CAT.

CONCLUSION

GPx1 (rs1050450), MnSOD (rs4880), and CAT (rs1001179) variants might not be a risk factor for PCOS.

摘要

目的

多囊卵巢综合征（PCOS）是一种常见的内分泌代谢紊乱疾病。有研究表明氧化应激与PCOS易感性及其代谢关联有关。本研究首次旨在探讨GPx1（rs1050450）、MnSOD（rs4880）和过氧化氢酶（rs1001179）基因变异对PCOS易感性的影响。

方法

在一项病例对照研究中，对来自伊朗西部的350名库尔德女性志愿者（175例PCOS患者和175名健康对照）进行了研究。采用PCR-RFLP法对GPx1和MnSOD进行基因分型，采用等位基因特异性PCR法对CAT进行基因分型。

结果

PCOS患者中多毛症、痤疮和黑棘皮病患者的比例分别为44.6%、30.3%和14.9%。PCOS患者与健康女性之间的等位基因分布情况为：Gpx1的“Pro”（69.1%对68.8%）和“Leu”（31.4%对31.2%），MnSOD的“Ala”（61.43%对56.57%）和“Val”（38.57%对43.43%），CAT的“C”（83.43%对84.57%）和“T”（16.57%对15.43%）。