采用高发光核碱基 dG-tC FRET 对研究核小体结构的方法。
Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase dG-tC FRET Pair.
机构信息
Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.
出版信息
Chemistry. 2018 Nov 16;24(64):17091-17095. doi: 10.1002/chem.201803382. Epub 2018 Oct 24.
A distance- and orientation-factor-dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobase dG-tC FRET pair to 601 nucleosomes is reported. It was found that the dG-tC FRET pair was successfully incorporated to 145 bp 601 sequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.
一种依赖于距离和取向因子的 FRET 系统是研究核小体构象动力学的一种有用且有吸引力的方法。在本研究中，报告了将高发光核碱基 dG-tC FRET 对应用于 601 核小体。结果发现，dG-tC FRET 对成功地掺入到 145bp 的 601 序列中，并在核小体中指定的供体和受体位置获得了不同的 FRET 效率。
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