Li Yuan, Zhang Ai-li, Li Guo-dong, Qian Zi-gang
Zhong Yao Cai. 2016 Sep;39(9):1971-4.
To establish a real-time quantitative PCR method to detect Psammosilene tunicoides β-actin, and to provide a reference gene for the detection of Psammosilene tunicoides genes by q PCR.
Specific primers were designed based on the conserved region of the β-actin gene( Gen Bank) and were used to amplify β-actin by PCR. β-actin was also used as a reference gene in the q PCR analysis of glycosyltransferase gene( UGT) expression in the roots,stems,and leaves of Psammosilene tunicoides.
The length of the β-actin gene amplicon from Psammosilene tunicoides was 153 bp and shared relatively high homology with β-actin found in Vaccaria segetalis, Myosoton aquaticum and Portulaca oleracea. Furthermore, UGT was revealed to be stably expressed in different Psammosilene tunicoides tissues when β-actin was employed as the reference gene.
β-actin is a reliable and suitable reference gene for studies on the expression of triterpenoid saponin biosynthesis-related genes in Psammosilene tunicoides.
建立一种实时定量PCR方法来检测金铁锁β-肌动蛋白，并为通过qPCR检测金铁锁基因提供一个内参基因。
基于β-肌动蛋白基因（GenBank）的保守区域设计特异性引物，用于通过PCR扩增β-肌动蛋白。β-肌动蛋白还被用作金铁锁根、茎和叶中糖基转移酶基因（UGT）表达的qPCR分析中的内参基因。
金铁锁β-肌动蛋白基因扩增子长度为153bp，与王不留行、小无心菜和马齿苋中的β-肌动蛋白具有较高的同源性。此外，当以β-肌动蛋白作为内参基因时，UGT在金铁锁不同组织中稳定表达。
β-肌动蛋白是用于金铁锁三萜皂苷生物合成相关基因表达研究的可靠且合适的内参基因。
