Yue Xiu, Liu Zhuhan, Yu Guangping, Li Qianhua, Tang Jiali
Shenyang Institute of Automation in Guangzhou, Chinese Academy of Sciences, Guangzhou 511458, China E-mail:
Water Sci Technol. 2018 Sep;78(3-4):515-522. doi: 10.2166/wst.2018.316.
Stable performance is a technical problem in the completely autotrophic nitrogen removal over nitrite (CANON) process with one single stage, which needs to be addressed. In the current work, a laboratory-scale submerged aerated biological filter (SABF) with a 3-L working volume was introduced into the CANON process to enhance its stable performance for 290 days under the following conditions: temperature of 30 ± 1 °C and dissolved oxygen (DO) level of 0.2-0.8 mg·L. The results showed that the average ammonium nitrogen removal efficiencies (ANRE) and total nitrogen removal efficiencies (TNRE) were 97.4% and 75.7%, respectively. A 16S rRNA gene high-throughput sequencing technology confirmed the phyla Proteobacteria and Planctomycetes as the ammonium oxidizing bacteria (AOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) of this CANON process with SABF, respectively. The major contributor to nitrogen removal was the genus Candidatus Brocadia, in Brocadiae. The aim is to present an effective strategy as a reference for the design of full-scale plant for the CANON process.
稳定性能是单级亚硝酸盐完全自养脱氮（CANON）工艺中的一个技术问题，需要加以解决。在当前工作中，将一个工作体积为3升的实验室规模的淹没式曝气生物滤池（SABF）引入CANON工艺，在温度为30±1°C、溶解氧（DO）水平为0.2 - 0.8 mg·L的条件下，提高其稳定性能达290天。结果表明，平均氨氮去除效率（ANRE）和总氮去除效率（TNRE）分别为97.4%和75.7%。16S rRNA基因高通量测序技术证实，变形菌门和浮霉菌门分别是该带有SABF的CANON工艺中的氨氧化细菌（AOB）和厌氧氨氧化细菌（AnAOB）。脱氮的主要贡献者是布罗卡德氏菌属中的“暂定布罗卡德氏菌”。目的是提出一种有效策略，为CANON工艺的全尺寸工厂设计提供参考。
