Aitasalo K
Acta Radiol Oncol. 1986 May-Jun;25(3):207-12. doi: 10.3109/02841868609136407.
The influence of irradiation was studied histochemically in healing mandibular periosteum and bone. After a cut line had been made on both sides of the mandible the rats were exposed to roentgen ray irradiation. The single doses were 15, 20, 30, 35 or 40 Gy. The animals were killed 1, 2, 4, 8, 10, 12, 16 and 24 hours after irradiation, for histochemical analysis. All enzymes, acid phosphatase, cytochrome oxidase, lactate, isocitrate, glucose-6-phosphatase and succinate dehydrogenase, showed a greater increase in enzyme staining in the irradiated cut lines than in the non-irradiated control lines. The intensity of the staining increased with time and dose over 24 hours. The observation time included an inflammatory phase with vascular, enzymatic and cellular responses to periosteal and bone injury. The increase in staining was dependent on the time after surgical trauma and radiation dose. The increase in enzyme staining probably represents the initial cell damage after irradiation.
通过组织化学方法研究了辐射对愈合期下颌骨骨膜和骨骼的影响。在大鼠下颌骨两侧制造切割线后，对其进行X射线照射。单次剂量分别为15、20、30、35或40 Gy。在照射后1、2、4、8、10、12、16和24小时处死动物，进行组织化学分析。所有酶，即酸性磷酸酶、细胞色素氧化酶、乳酸、异柠檬酸、葡萄糖-6-磷酸酶和琥珀酸脱氢酶，在照射后的切割线处酶染色的增加幅度均大于未照射的对照线。在24小时内，染色强度随时间和剂量增加。观察时间包括对骨膜和骨损伤产生血管、酶和细胞反应的炎症期。染色增加取决于手术创伤后的时间和辐射剂量。酶染色增加可能代表照射后的初始细胞损伤。
