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利用酶联免疫吸附测定、血凝反应和电子显微镜技术检测水貂粪便中的水貂肠炎病毒。

Detection of mink enteritis virus in mink feces, using enzyme-linked immunosorbent assay, hemagglutination, and electron microscopy.

作者信息

Shen D T, Ward A C, Gorham J R

出版信息

Am J Vet Res. 1986 Sep;47(9):2025-30.

PMID:3021033
Abstract

Twenty-five mink were inoculated with mink enteritis virus (MEV). Fecal specimens were collected daily and were simultaneously evaluated for MEV antigen by use of a direct enzyme-linked immunosorbent assay (ELISA), hemagglutination (HA), and electron microscopy. Results of the evaluations indicated that MEV was shed in the feces on postinoculation days 5 and 6. The virus was not detectable by ELISA or HA after postinoculation day 6, although viruses were found in reduced numbers by use of electron microscopy. The ELISA was specific for MEV, and the sensitivity of the ELISA for MEV was comparable with that of HA.

摘要

给25只水貂接种水貂肠炎病毒(MEV)。每天收集粪便样本,并同时使用直接酶联免疫吸附测定(ELISA)、血凝试验(HA)和电子显微镜对MEV抗原进行评估。评估结果表明,接种后第5天和第6天粪便中排出了MEV。接种后第6天之后,ELISA或HA检测不到该病毒,不过通过电子显微镜发现病毒数量有所减少。ELISA对MEV具有特异性,其对MEV的敏感性与HA相当。

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引用本文的文献

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Development of a nanoparticle-assisted PCR (nanoPCR) assay for detection of mink enteritis virus (MEV) and genetic characterization of the NS1 gene in four Chinese MEV strains.一种纳米粒子辅助 PCR(nanoPCR)检测方法的建立及其在中国 4 株貂肠炎病毒(MEV)中 NS1 基因的遗传特征分析。
BMC Vet Res. 2015 Jan 13;11:1. doi: 10.1186/s12917-014-0312-6.