Grino M, Castanas E, Conte-Devolx B, Guillaume V, Boudouresque F, Oliver C
Ann Endocrinol (Paris). 1986;47(1):51-3.
Specific binding sites for rat CRF (r-CRF) have been characterized on rat anterior pituitary membranes. The binding of the radioiodinated analog of r-CRF (125I Tyr-r-CRF) was time, temperature, pH and protein dependent. No interaction was found with other neurohormones except with Arginine Vasopressin, but at supra physiological levels. Two classes of specific binding sites (high affinity and low affinity) for r-CRF were identified. Bilateral adrenalectomy provoked, since the 24th hour and up to 7 days, in addition of an increase of ACTH plasmatic levels, an abolition of the high affinity binding site; corticosterone treatment reversed these changes. This finding suggests that circulating glucocorticoids may control the anterior pituitary binding sites for CRF, either by a direct action on the anterior pituitary, or by a modulatory effect on hypothalamic CRF secretion.
大鼠促肾上腺皮质激素释放因子（r-CRF）的特异性结合位点已在大鼠垂体前叶膜上得到表征。r-CRF放射性碘化类似物（125I Tyr-r-CRF）的结合具有时间、温度、pH和蛋白质依赖性。除了与精氨酸加压素在超生理水平上有相互作用外，未发现与其他神经激素有相互作用。已鉴定出两类r-CRF特异性结合位点（高亲和力和低亲和力）。双侧肾上腺切除术后，从第24小时直至7天，除了促肾上腺皮质激素血浆水平升高外，高亲和力结合位点消失；皮质酮治疗可逆转这些变化。这一发现表明，循环糖皮质激素可能通过对垂体前叶的直接作用或对下丘脑CRF分泌的调节作用来控制垂体前叶CRF的结合位点。
