Hu Xinyuan, Tandra Nitin, Zhang Zhijian, Gong Aihua, Chen Jingyan, Li Yang, Chen Qian, Xu Wenrong, Qian Hui
Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and Transformation Application, Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University Zhenjiang, People's Republic of China.
Am J Transl Res. 2018 Aug 15;10(8):2619-2627. eCollection 2018.
Pterygium is an invasive hyperplasia, resulting from the hyper-proliferation of epithelial cells in cornea. The aim of our present study is to identify stem cells derived from pterygiums and detect differentiation strategy of the pterygium stem cells in vitro. Fundamental properties of the cultured pterygium stem cells were mainly studied using immunofluorescence staining. While clone formation and MTT assay were utilized to evaluate the cell viability. Pterygium stem cells could be cultivated easily, expanded efficiently, but expressed multilineage stem cell markers, which could be differentiated into neuron, osteocytes and adipocytes in vitro. Pterygium-derived spheres expressed stem cell markers and the epithelial-mesenchymal transition markers after treating with the inducing assays. Proliferation and viability of pterygium stem cells could be inhibited after inducing differentiation indicating that differentiation strategy will be a promising strategy in future therapies of pterygium. Cells derived from pterygiums express multilineage stem cell markers and could be induced differentiation. Differentiation therapy strategy could inhibit pterygium stem cell in vitro.
翼状胬肉是一种侵袭性增生，由角膜上皮细胞的过度增殖引起。我们当前研究的目的是鉴定源自翼状胬肉的干细胞，并检测翼状胬肉干细胞在体外的分化策略。主要使用免疫荧光染色研究培养的翼状胬肉干细胞的基本特性。同时利用克隆形成和MTT分析来评估细胞活力。翼状胬肉干细胞易于培养、高效扩增，但表达多谱系干细胞标志物，在体外可分化为神经元、骨细胞和脂肪细胞。在用诱导分析处理后，源自翼状胬肉的球体表达干细胞标志物和上皮-间质转化标志物。诱导分化后，翼状胬肉干细胞的增殖和活力可被抑制，这表明分化策略将是未来翼状胬肉治疗中的一种有前景的策略。源自翼状胬肉的细胞表达多谱系干细胞标志物且可被诱导分化。分化治疗策略可在体外抑制翼状胬肉干细胞。
