Huang FengFei, Ben Aissa Manel, Lévesque Georges, Carreau Madeleine
Centre Hospitalier Universitaire de Québec-Université Laval, CHUL, 2705 Boul. Laurier, RC-9800, Quebec, QC, G1V 4G2, Canada.
Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Chicago, IL, USA.
BMC Res Notes. 2018 Sep 12;11(1):662. doi: 10.1186/s13104-018-3763-1.
The Uncoordinated 5A (UNC5A) protein is part of a family of receptors that play roles in axonal pathfinding and cell migration. We previously showed that the Fanconi anemia C protein (FANCC) interacts with UNC5A and delays UNC5A-mediated apoptosis. FANCC is a predominantly cytoplasmic protein that has multiple functions including DNA damage signaling, oxygen radical metabolism, signal transduction, transcriptional regulation and apoptosis. Given the direct interaction between FANCC and UNC5A and that FANCC interferes with UNC5A-mediated apoptosis, we explored the possibility that FANCC might play a role in axonal-like growth processes.
Here we show that FANCC and UNC5A are localized to regions of neurite outgrowth during neuronal cell differentiation. We also show that absence of FANCC is required for neurite outgrowth. In addition, FANCC seems required for UNC5A expression. Results from this study combined with our previous report suggest that FANCC plays a role in tissue development through the regulation of UNC5A-mediated functions.
不协调5A（UNC5A）蛋白是受体家族的一部分，在轴突导向和细胞迁移中发挥作用。我们之前表明，范可尼贫血C蛋白（FANCC）与UNC5A相互作用，并延迟UNC5A介导的细胞凋亡。FANCC是一种主要位于细胞质的蛋白，具有多种功能，包括DNA损伤信号传导、氧自由基代谢、信号转导、转录调控和细胞凋亡。鉴于FANCC与UNC5A之间的直接相互作用以及FANCC干扰UNC5A介导的细胞凋亡，我们探讨了FANCC可能在轴突样生长过程中发挥作用的可能性。
在此我们表明，在神经元细胞分化过程中，FANCC和UNC5A定位于神经突生长区域。我们还表明，神经突生长需要缺失FANCC。此外，FANCC似乎是UNC5A表达所必需的。本研究结果与我们之前的报告相结合表明，FANCC通过调节UNC5A介导的功能在组织发育中发挥作用。
