Klahs Phillip C, Gallaher Timothy J, Clark Lynn G
Department of Ecology, Evolution, and Organismal Biology Iowa State University 2200 Osborn Drive Ames Iowa 50011 USA.
Department of Biology University of Washington Box 351800 Seattle Washington 98195-1800 USA.
Appl Plant Sci. 2018 Aug 27;6(8):e01177. doi: 10.1002/aps3.1177. eCollection 2018 Aug.
A refined procedure is described for modeling small, intricate plant structures using computer-aided design software. The procedure facilitates the study of wind pollination in the family Poaceae and provides virtual biological illustrations for public outreach.
Spikelets were fixed in gFAA, dehydrated using ethanol and xylene, embedded in paraffin wax, and then sectioned with a rotary microtome. Images of serial sections were used as a reference for modeling the shape of bracts with splines in a computer-aided design program. Virtual models produced by this method have many potential uses; examples include geometric morphometric analyses and simulations of computational fluid dynamics.
This protocol is a synthesis of modern biological illustration and engineering technology. Virtual models facilitate quantitative experiments that may address questions about reproductive biology, conditions shaping the form of anatomical support, or the morphological evolution of structures of biomechanical interest.
描述了一种使用计算机辅助设计软件对小型复杂植物结构进行建模的精细程序。该程序有助于对禾本科植物的风媒传粉进行研究，并为公众推广提供虚拟生物插图。
将小穗固定在gFAA中，用乙醇和二甲苯脱水，嵌入石蜡，然后用旋转切片机切片。连续切片的图像用作在计算机辅助设计程序中用样条对苞片形状进行建模的参考。通过这种方法生成的虚拟模型有许多潜在用途；例如几何形态计量分析和计算流体动力学模拟。
该方案是现代生物插图和工程技术的综合。虚拟模型有助于进行定量实验，这些实验可以解决有关生殖生物学、塑造解剖学支撑形式的条件或具有生物力学意义的结构的形态演化等问题。
