Department of Animal Science, Faculty of Agrarian and Veterinary Sciences, Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
Department of Animal Science, Faculty of Agrarian and Veterinary Sciences, Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
Mol Cell Probes. 2018 Oct;41:39-42. doi: 10.1016/j.mcp.2018.09.001. Epub 2018 Sep 12.
This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs.
本研究通过研究与乳腺炎抗性和易感性表型相关的免疫反应机制，比较了候选基因 CSF3 和 LPO 的表达谱。使用定量实时 PCR (qRT-PCR)技术，在 24 份有乳腺炎（N=12）和无乳腺炎（N=12）的水牛牛奶样本中，测定了粒细胞集落刺激因子 3 (CSF3) 和乳过氧化物酶 (LPO)基因的表达谱。与健康动物相比，患有乳腺炎的动物中 CSF3 和 LPO 的表达分别高出 5.14 倍（P=0.001）和 2.41 倍（P=0.097），表明这两个基因在研究组中存在不同的表达趋势。我们的发现表明，LPO 和 CSF3 基因是奶牛抵抗乳腺炎的重要防御机制，并且可能是在水牛繁殖计划中选择更健康动物的候选基因。
