Medical Experiment Center, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 314 An Shan Xi Road, Nan Kai District, Tianjin, 300193, China.
Tianjin University of Traditional Chinese Medicine, 312 An Shan Xi Road, Nan Kai District, Tianjin, 300193, China.
Inflammation. 2019 Feb;42(1):264-275. doi: 10.1007/s10753-018-0891-7.
TanshinoneIIA (TanIIA) has been demonstrated to possess numerous biological effects. However, the specific effect of TanIIA on macrophage polarization has not been reported. In this study, it was revealed that TanIIA might play a pivotal role in macrophage polarization. As our results indicated, cell morphology was changed in RAW264.7 cells which were treated with LPS or LPS/TanIIA (0.1 μM, 1 μM, 10 μM). Subsequently, pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 were measured by ELISA kits. Furthermore, TanIIA enhanced the expression of M2 macrophage markers (Arg1 and FIZZ1) and decreased the expression of markers associated with M1 macrophage polarization (iNOS and IL-1β). Increased expression of CD206 was also detected by flow cytometry in TanIIA-treated groups. Mechanistically, it was revealed that TanIIA modulated macrophage polarization by ameliorating mitochondrial function and regulating TLR4-HMGB1/CEBP-β pathway. In addition, increased expression of miR-155 was observed in RAW264.7 cells incubated with LPS and were effectively inhibited by TanIIA. Taken together, it was suggested that TanIIA inhibits inflammatory response and promotes macrophage polarization toward an M2 phenotype, which provides new evidence for the anti-inflammation activity of TanIIA.
丹参酮 IIA（TanIIA）具有多种生物学效应。然而，TanIIA 对巨噬细胞极化的具体作用尚未见报道。在本研究中，揭示了 TanIIA 可能在巨噬细胞极化中发挥关键作用。我们的结果表明，LPS 或 LPS/TanIIA（0.1μM、1μM、10μM）处理 RAW264.7 细胞后，细胞形态发生改变。随后，通过 ELISA 试剂盒测量促炎细胞因子 TNF-α和抗炎细胞因子 IL-10。此外，TanIIA 增强了 M2 巨噬细胞标志物（Arg1 和 FIZZ1）的表达，并降低了与 M1 巨噬细胞极化相关的标志物（iNOS 和 IL-1β）的表达。通过流式细胞术检测到 TanIIA 处理组中 CD206 的表达也增加。在机制上，发现 TanIIA 通过改善线粒体功能和调节 TLR4-HMGB1/CEBP-β途径来调节巨噬细胞极化。此外，在孵育有 LPS 的 RAW264.7 细胞中观察到 miR-155 的表达增加，并且 TanIIA 可有效抑制其表达。综上所述，TanIIA 抑制炎症反应并促进巨噬细胞向 M2 表型极化，为 TanIIA 的抗炎活性提供了新的证据。
