Inoue Kimiko, Matoba Shogo, Ogura Atsuo
RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2018;1861:55-65. doi: 10.1007/978-1-4939-8766-5_5.
Somatic cell nuclear transfer (SCNT) enables the production of animals from single cell nuclei. Unlike normally fertilized embryos, SCNT-derived embryos ectopically express the Xist gene from the maternal allele, because of the lack of Xist-repressing imprints in the somatic donor genome. This has severely compromised the development of SCNT-derived embryos, at least in mice. Here, we describe the basic protocol of mouse SCNT as well as a Xist knockdown SCNT procedure, which remarkably increases the efficiency of cloning mice.
体细胞核移植（SCNT）能够从单个细胞核培育出动物。与正常受精的胚胎不同，由于体细胞核供体基因组中缺乏抑制Xist基因的印记，SCNT衍生胚胎会从母本等位基因异位表达Xist基因。这严重阻碍了SCNT衍生胚胎的发育，至少在小鼠中是这样。在这里，我们描述了小鼠SCNT的基本方案以及Xist基因敲低的SCNT程序，该程序显著提高了克隆小鼠的效率。
