减数分裂性染色体失活的染色体铺展分析
Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation.
作者信息
Alavattam Kris G, Abe Hironori, Sakashita Akihiko, Namekawa Satoshi H
机构信息
Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2018;1861:113-129. doi: 10.1007/978-1-4939-8766-5_10.
Abstract
A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.
摘要
一种独特的X染色体失活形式发生在雄性减数分裂过程中，此时雄性性染色体经历一种称为减数分裂性染色体失活（MSCI）的现象。MSCI由独立于Xist RNA的DNA损伤反应信号传导引导，以沉默性染色体的转录活性，这是雄性生殖细胞发育中的一个重要事件。在这里，我们展示了用于制备和分析小鼠减数分裂精母细胞染色体铺展玻片的方案，从而提供一种快速、廉价且强大的细胞学方法来补充基因表达研究。
相似文献
1
Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation.减数分裂性染色体失活的染色体铺展分析
Methods Mol Biol. 2018;1861:113-129. doi: 10.1007/978-1-4939-8766-5_10.
2
Incomplete meiotic sex chromosome inactivation in the domestic dog.家犬减数分裂性染色体不完全失活
BMC Genomics. 2015 Apr 12;16(1):291. doi: 10.1186/s12864-015-1501-9.
3
Extensive meiotic asynapsis in mice antagonises meiotic silencing of unsynapsed chromatin and consequently disrupts meiotic sex chromosome inactivation.小鼠中广泛的减数分裂联会异常对抗未联会染色质的减数分裂沉默，从而破坏减数分裂性染色体失活。
J Cell Biol. 2008 Jul 28;182(2):263-76. doi: 10.1083/jcb.200710195.
4
UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice†.UHRF1 对于减数分裂性染色体失活是不可或缺的，并且在小鼠中与 DNA 损伤反应途径相互作用†。
Biol Reprod. 2022 Jul 25;107(1):168-182. doi: 10.1093/biolre/ioac054.
5
Escape of X-linked miRNA genes from meiotic sex chromosome inactivation.X连锁miRNA基因从减数分裂性染色体失活中逃逸。
Development. 2015 Nov 1;142(21):3791-800. doi: 10.1242/dev.127191. Epub 2015 Sep 22.
6
Meiotic sex chromosome inactivation in male mice with targeted disruptions of Xist.Xist基因靶向破坏的雄性小鼠减数分裂中的性染色体失活
J Cell Sci. 2002 Nov 1;115(Pt 21):4097-105. doi: 10.1242/jcs.00111.
7
Human male meiotic sex chromosome inactivation.人类雄性减数分裂性染色体失活。
PLoS One. 2012;7(2):e31485. doi: 10.1371/journal.pone.0031485. Epub 2012 Feb 15.
8
Zfy genes are required for efficient meiotic sex chromosome inactivation (MSCI) in spermatocytes.Zfy基因是精母细胞中有效减数分裂性染色体失活（MSCI）所必需的。
Hum Mol Genet. 2016 Dec 15;25(24):5300-5310. doi: 10.1093/hmg/ddw344.
9
Expression and epigenomic landscape of the sex chromosomes in mouse post-meiotic male germ cells.小鼠减数分裂后雄性生殖细胞中性染色体的表达及表观基因组景观
Epigenetics Chromatin. 2016 Oct 27;9:47. doi: 10.1186/s13072-016-0099-8. eCollection 2016.
10
H2AX is required for chromatin remodeling and inactivation of sex chromosomes in male mouse meiosis.在雄性小鼠减数分裂过程中，染色质重塑和性染色体失活需要H2AX。
Dev Cell. 2003 Apr;4(4):497-508. doi: 10.1016/s1534-5807(03)00093-5.
引用本文的文献
1
Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis.Pdha2对X连锁的Pdha1沉默的补偿对于精子发生过程中的减数分裂双链断裂修复至关重要。
Development. 2025 Aug 1;152(15). doi: 10.1242/dev.204683. Epub 2025 Aug 7.
2
Antibody Labeling of Mouse Spermatocyte Chromosome Spreads to Study Meiosis.小鼠精母细胞染色体铺展的抗体标记用于研究减数分裂
Methods Mol Biol. 2025;2954:199-206. doi: 10.1007/978-1-0716-4698-4_10.
3
CTCF-mediated 3D chromatin sets up the gene expression program in the male germline.CTCF介导的三维染色质构建雄性生殖系中的基因表达程序。
Nat Struct Mol Biol. 2025 Mar 3. doi: 10.1038/s41594-025-01482-z.
4
hnRNPC Functions with HuR to Regulate Alternative Splicing in an m6A-Dependent Manner and is Essential for Meiosis.异质性核糖核蛋白C（hnRNPC）与HuR共同发挥作用，以m6A依赖的方式调节可变剪接，并且对减数分裂至关重要。
Adv Sci (Weinh). 2025 Apr;12(13):e2412196. doi: 10.1002/advs.202412196. Epub 2025 Feb 8.
5
METTL16 is Required for Meiotic Sex Chromosome Inactivation and DSB Formation and Recombination during Male Meiosis.减数分裂性染色体失活以及雄性减数分裂期间双链断裂形成和重组需要METTL16
Adv Sci (Weinh). 2025 Jan;12(3):e2406332. doi: 10.1002/advs.202406332. Epub 2024 Nov 28.
6
Meiotic chromatin-associated HSF5 is indispensable for pachynema progression and male fertility.减数分裂染色质相关的 HSF5 对于粗线期进展和雄性育性是必不可少的。
Nucleic Acids Res. 2024 Sep 23;52(17):10255-10275. doi: 10.1093/nar/gkae701.
7
A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation.一种导致男性不育的 TOPBP1 等位基因使 XY 沉默动力学与性体形成脱耦。
Elife. 2024 Feb 23;12:RP90887. doi: 10.7554/eLife.90887.
8
ATF7IP2/MCAF2 directs H3K9 methylation and meiotic gene regulation in the male germline.ATF7IP2/MCAF2 指导雄性生殖细胞中 H3K9 甲基化和减数分裂基因调控。
Genes Dev. 2024 Mar 22;38(3-4):115-130. doi: 10.1101/gad.351569.124.
9
ATF7IP2/MCAF2 directs H3K9 methylation and meiotic gene regulation in the male germline.ATF7IP2/MCAF2指导雄性生殖系中的H3K9甲基化和减数分裂基因调控。
bioRxiv. 2023 Oct 2:2023.09.30.560314. doi: 10.1101/2023.09.30.560314.
10
Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation.活跃的DNA损伤反应信号传导启动并维持减数分裂性染色体失活。
Nat Commun. 2022 Nov 28;13(1):7212. doi: 10.1038/s41467-022-34295-5.
本文引用的文献
1
Elucidation of the Fanconi Anemia Protein Network in Meiosis and Its Function in the Regulation of Histone Modifications.减数分裂中范可尼贫血蛋白网络的解析及其在组蛋白修饰调控中的作用
Cell Rep. 2016 Oct 18;17(4):1141-1157. doi: 10.1016/j.celrep.2016.09.073.
2
FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.FANCB在雄性生殖细胞系中至关重要，并在减数分裂过程中调节性染色体上的H3K9甲基化。
Hum Mol Genet. 2015 Sep 15;24(18):5234-49. doi: 10.1093/hmg/ddv244. Epub 2015 Jun 29.
3
BAZ1B is dispensable for H2AX phosphorylation on Tyrosine 142 during spermatogenesis.在精子发生过程中，BAZ1B对于H2AX在酪氨酸142位点的磷酸化并非必需。
Biol Open. 2015 May 15;4(7):873-84. doi: 10.1242/bio.011734.
4
Flow cytometry of murine spermatocytes.小鼠精母细胞的流式细胞术
Curr Protoc Cytom. 2015 Apr 1;72:7.44.1-7.44.24. doi: 10.1002/0471142956.cy0744s72.
5
SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.SCML2 通过调节组蛋白 H2A 的泛素化来建立雄性生殖系表观基因组。
Dev Cell. 2015 Mar 9;32(5):574-88. doi: 10.1016/j.devcel.2015.01.014. Epub 2015 Feb 19.
6
BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis.BRCA1在雄性减数分裂中建立DNA损伤信号和X染色体的着丝粒周围异染色质。
J Cell Biol. 2014 Jun 9;205(5):663-75. doi: 10.1083/jcb.201311050.
7
Optimized flow cytometry isolation of murine spermatocytes.小鼠精母细胞的优化流式细胞术分离法
Cytometry A. 2014 Jun;85(6):556-65. doi: 10.1002/cyto.a.22463. Epub 2014 Mar 24.
8
Slide preparation method to preserve three-dimensional chromatin architecture of testicular germ cells.保留睾丸生殖细胞三维染色质结构的玻片制备方法。
J Vis Exp. 2014 Jan 10(83):e50819. doi: 10.3791/50819.
9
RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids.RNF8 调节有丝分裂后精子细胞中活跃的表观遗传修饰和从失活性性染色体上逃避基因激活。
Genes Dev. 2012 Dec 15;26(24):2737-48. doi: 10.1101/gad.202713.112.
10
Sex chromosome inactivation in germ cells: emerging roles of DNA damage response pathways.生殖细胞中的性染色体失活：DNA 损伤反应途径的新作用。
Cell Mol Life Sci. 2012 Aug;69(15):2559-72. doi: 10.1007/s00018-012-0941-5. Epub 2012 Mar 2.
Zotero 插件