减数分裂性染色体失活的染色体铺展分析
Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation.
作者信息
Alavattam Kris G, Abe Hironori, Sakashita Akihiko, Namekawa Satoshi H
机构信息
Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2018;1861:113-129. doi: 10.1007/978-1-4939-8766-5_10.
Abstract
A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.
摘要
一种独特的X染色体失活形式发生在雄性减数分裂过程中，此时雄性性染色体经历一种称为减数分裂性染色体失活（MSCI）的现象。MSCI由独立于Xist RNA的DNA损伤反应信号传导引导，以沉默性染色体的转录活性，这是雄性生殖细胞发育中的一个重要事件。在这里，我们展示了用于制备和分析小鼠减数分裂精母细胞染色体铺展玻片的方案，从而提供一种快速、廉价且强大的细胞学方法来补充基因表达研究。
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