一种用于测量肝素后血浆中脂蛋白脂肪酶和肝甘油三酯脂肪酶活性的自动化方法。
An automated method for measuring lipoprotein lipase and hepatic triglyceride lipase activities in post-heparin plasma.
机构信息
Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Imamura Enzyme Technology Laboratory, Shizuoka, Japan.
出版信息
Clin Chim Acta. 2018 Dec;487:54-59. doi: 10.1016/j.cca.2018.09.022. Epub 2018 Sep 12.
BACKGROUND
Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) play a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of LPL and HTGL activity is useful for diagnosing lipid disorders, but there has been no automated method for measuring these lipase activities.
METHODS
The automated kinetic colorimetric method was used for assaying LPL and HTGL activity in the post-heparin plasma using the natural long-chain fatty acid 2-diglyceride as a substrate. LPL activity was determined with apoCII and HTGL activity was determined without apoCII with 2 channel of auto-analyzer.
RESULTS
The calibration curve for dilution tests of the LPL and HTGL activity assay ranged from 0.0 to 500 U/L. Within-run CV was obtained within a range of 5%. No interference was observed in the testing of specimens containing potentially interfering substances. The measurement range of LPL activity in the post-heparin plasma was 30-153 U/L, while HTGL activity was 135-431 U/L in normal controls.
CONCLUSIONS
The L PL and HTGL activity assays are applicable to quantitating the LPL and HTGL activity in the post-heparin plasma. This assay is more convenient and faster than radiochemical assay and highly suitable for the detection of lipid disorders.
背景
脂蛋白脂肪酶 (LPL) 和肝甘油三酯脂肪酶 (HTGL) 通过催化甘油三酯水解，在富含甘油三酯的脂蛋白代谢中发挥核心作用。定量测定 LPL 和 HTGL 的活性有助于诊断脂质代谢紊乱，但目前还没有用于测量这些脂肪酶活性的自动化方法。
方法
使用天然长链脂肪酸 2-二甘油酯作为底物，通过自动生化分析仪的双通道，采用酶动力学比色法检测肝素后血浆中 LPL 和 HTGL 的活性。LPL 活性测定时加入载脂蛋白 CII（apoCII），HTGL 活性测定时不加入 apoCII。
结果
LPL 和 HTGL 活性测定的稀释试验校准曲线范围为 0.0-500U/L。批内 CV 在 5%范围内。在测试含有潜在干扰物质的标本时未观察到干扰。肝素后血浆中 LPL 活性的测定范围为 30-153U/L，正常对照者 HTGL 活性为 135-431U/L。
结论
LPL 和 HTGL 活性测定可用于定量检测肝素后血浆中的 LPL 和 HTGL 活性。与放射性化学测定法相比，该测定法更方便、更快，非常适合检测脂质代谢紊乱。
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