Zhang Shu, Yuan Ming, Cheng Cheng, Xia Dong-Hui, Wu Shi-Wen
Aerosp Med Hum Perform. 2018 Oct 1;89(10):883-888. doi: 10.3357/AMHP.5079.2018.
Skeletal muscle atrophy is a striking example of the multiple changes in the physiological state of humans and animals induced by microgravity. Previous studies have shown that a blood circulation disorder may be a cause of this atrophy, and traditional Chinese medicine has been regarded as a potential countermeasure to reverse the atrophy in China. This study was carried out to test the effects of Xuefuzhuyu capsules (XFZY) on the skeletal muscle atrophy induced by simulated microgravity.
The mass and cross-sectional area of the soleus muscle were compared in rats treated with XFZY that were hindlimb unloaded for 30 d (XFZY-TS group), untreated rats that were hindlimb unloaded for 30 d (TS group), and control rats (CON group). The expression and phosphorylation levels of key proteins of the sarcoplasmic reticulum stress system were also measured.
Treatment with XFZY attenuated the loss of muscle mass and cross-sectional area induced by hindlimb unloading. Western blot analysis showed that the phosphatidyl-inositol-3-kinase/phospho-Akt (PI3K/p-Akt) pathways were down-regulated after 30 d in the TS group compared with the CON group. This effect was partly reversed by XFZY. Hindlimb unloading increased the expression of glucose-regulated protein 78 (GRP78), cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein homologous protein (CHOP), C-Jun N-terminal kinase (JNK), and Caspase 12. Treatment with XFZY alleviated this increased protein expression.
Our results suggest that XFZY could partially reverse the effects of hindlimb unloading on muscle atrophy and perhaps target the sarcoplasmic reticulum stress system, possibly through the GRP78-CHOP-JNK-Caspase 12 pathway.Zhang S, Yuan M, Cheng C, Xia D, Wu S. Chinese herbal medicine effects on muscle atrophy induced by simulated microgravity. Aerosp Med Hum Perform. 2018; 89(10):883-888.
骨骼肌萎缩是微重力诱导的人类和动物生理状态多种变化的一个显著例子。先前的研究表明，血液循环障碍可能是这种萎缩的一个原因，而在我国，传统中药被视为逆转萎缩的一种潜在对策。本研究旨在测试血府逐瘀胶囊（XFZY）对模拟微重力诱导的骨骼肌萎缩的影响。
比较了后肢卸载30天的XFZY处理大鼠（XFZY-TS组）、后肢卸载30天的未处理大鼠（TS组）和对照大鼠（CON组）比目鱼肌的质量和横截面积。还测量了肌浆网应激系统关键蛋白的表达和磷酸化水平。
XFZY处理减轻了后肢卸载引起的肌肉质量和横截面积的损失。蛋白质印迹分析表明，与CON组相比，TS组30天后磷脂酰肌醇-3-激酶/磷酸化蛋白激酶B（PI3K/p-Akt）通路下调。XFZY部分逆转了这种作用。后肢卸载增加了葡萄糖调节蛋白78（GRP78）、胞嘧啶-胞嘧啶-腺苷-腺苷-胸腺嘧啶/增强子结合蛋白同源蛋白（CHOP）、C-Jun氨基末端激酶（JNK）和半胱天冬酶12的表达。XFZY处理减轻了这种蛋白质表达的增加。
我们的结果表明，XFZY可能部分逆转后肢卸载对肌肉萎缩的影响，并且可能通过GRP78-CHOP-JNK-半胱天冬酶12通路靶向肌浆网应激系统。张S，袁M，程C，夏D，吴S。中药对模拟微重力诱导的肌肉萎缩的影响。航空航天医学与人类表现。2018；89（10）：883-888。
