ryanodine 受体开放时间由关闭状态决定。
Ryanodine Receptor Open Times Are Determined in the Closed State.
机构信息
Department of Physiology and Biophysics, Rush University Medical Center, Chicago, Illinois.
Department of Physiology and Biophysics, Rush University Medical Center, Chicago, Illinois.
出版信息
Biophys J. 2018 Oct 2;115(7):1160-1165. doi: 10.1016/j.bpj.2018.08.025. Epub 2018 Sep 13.
Abstract
The ryanodine receptor (RyR) ion channel releases Ca from intracellular stores by conducting Ca but also by recruiting neighboring RyRs to open, as RyRs are activated by micromolar levels of cytosolic Ca. Using long single-RyR recordings of the cardiac isoform (RyR2), we conclude that Ca binding to the cytosolic face of RyR while the channel is closed determines the distribution of open times. This mechanism explains previous findings that RyR is not activated by its own fluxed Ca. Our measurements also bolster previous findings that luminal [Ca] can affect both the cytosolic activation and inactivation sites and that RyR has different gating modes for the same ionic conditions.
摘要
ryanodine 受体 (RyR) 离子通道通过传导 Ca 来释放细胞内储存的 Ca，还可以通过招募相邻的 RyR 来打开，因为 RyR 被细胞浆内 Ca 浓度的微摩尔水平激活。使用心脏同工型 (RyR2) 的长单个 RyR 记录，我们得出结论，当通道关闭时，Ca 与 RyR 的细胞浆面结合决定了开放时间的分布。这种机制解释了先前的发现，即 RyR 不会被其自身的钙流激活。我们的测量结果也支持了先前的发现，即腔 [Ca] 可以影响细胞浆激活和失活部位，并且 RyR 在相同的离子条件下具有不同的门控模式。
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