载液离子强度对流场-流分馏回收率的影响
Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation.
作者信息
Kowalkowski Tomasz, Sugajski Mateusz, Buszewski Bogusław
机构信息
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7, 87-100 Torun, Poland.
2Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland.
出版信息
Chromatographia. 2018;81(8):1213-1218. doi: 10.1007/s10337-018-3551-z. Epub 2018 Jun 14.
Asymmetrical flow field-flow fractionation (AF4) and hollow-fiber flow field-flow fractionation (HF5) are techniques widely used in analytical, industrial and biological analyses. The main problem in all AF4 and HF5 analyses is sample loss due to analyte-membrane interactions. In this work the impact of liquid carrier composition on latex nanoparticles (NPs) separation in water and two different concentrations of NHNO was studied. In AF4, a constant trend of decreasing the size of 60 and 121.9 nm particles induced by the ionic strength of the carrier liquid has been observed. In contrast, an agglomeration effect of the biggest 356 nm particles was observed when increasing ionic strength, which induced a significant drop of recovery to 35%. H5F provides better resolution and intensified peaks of NPs, but careful optimisation of system parameters is mandatory to obtain good separation.
非对称流场-流分级（AF4）和中空纤维流场-流分级（HF5）是广泛应用于分析、工业和生物分析的技术。在所有AF4和HF5分析中，主要问题是由于分析物与膜的相互作用导致的样品损失。在这项工作中，研究了液体载体组成对水中和两种不同浓度的NHNO中乳胶纳米颗粒（NPs）分离的影响。在AF4中，观察到由载液离子强度引起的60和121.9nm颗粒尺寸减小的恒定趋势。相反，当增加离子强度时，观察到最大的356nm颗粒的团聚效应，这导致回收率显著下降至35%。HF5提供了更好的分辨率和增强的NPs峰，但必须仔细优化系统参数才能获得良好的分离效果。
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