Higashiyama S, Ishiguro H, Ohkubo I, Fujimoto S, Matsuda T, Sasaki M
Life Sci. 1986 Nov 3;39(18):1639-44. doi: 10.1016/0024-3205(86)90160-8.
During the investigation of inhibitory activity of kininogens toward calpains [EC 3.4.22.17], we found that lysyl-bradykinin was liberated from both high molecular weight (HMW) and low molecular weight (LMW) kininogens by the action of the calpains. The kinin liberation occurred in a limited range of calpain to kininogen molar ratios of 0.5:1 to 8:1, and in that condition calpains were simultaneously inhibited 20 to 80% by kininogens. The maximum level of kinin release from HMW and LMW kininogens by calpain I was about 25% and that by calpain II was 20%. These results suggest that in case of inflammation the kininogens play two physiologically distinct roles by interaction with calpains: to release lysyl-bradykinin and to inhibit proteinase activity of calpains derived from the damaged tissues.
在研究激肽原对钙蛋白酶[EC 3.4.22.17]的抑制活性过程中，我们发现，在钙蛋白酶的作用下，高分子量（HMW）和低分子量（LMW）激肽原均会释放出赖氨酰缓激肽。激肽释放发生在钙蛋白酶与激肽原摩尔比为0.5:1至8:1的有限范围内，在此条件下，激肽原会同时使钙蛋白酶的活性受到20%至80%的抑制。钙蛋白酶I从HMW和LMW激肽原释放激肽的最大水平约为25%，钙蛋白酶II为20%。这些结果表明，在炎症情况下，激肽原通过与钙蛋白酶相互作用发挥两种生理上不同的作用：释放赖氨酰缓激肽以及抑制受损组织来源的钙蛋白酶的蛋白酶活性。
