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Chromatin 3'-phosphatase/5'-OH kinase cannot transfer phosphate from 3' to 5' across a strand nick in DNA.染色质3'-磷酸酶/5'-羟基激酶无法跨越DNA链上的切口将磷酸从3'端转移至5'端。
Nucleic Acids Res. 1986 Oct 24;14(20):8103-10. doi: 10.1093/nar/14.20.8103.
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本文引用的文献

1
Sites and structure of gamma radiation-induced DNA strand breaks.γ辐射诱导的DNA链断裂的位点与结构。
J Biol Chem. 1982 Oct 10;257(19):11750-4.
2
The DNA 3'-phosphatase and 5'-hydroxyl kinase of rat liver chromatin.大鼠肝脏染色质的DNA 3'-磷酸酶和5'-羟基激酶
FEBS Lett. 1983 Aug 22;160(1-2):46-50. doi: 10.1016/0014-5793(83)80933-8.
3
3'-Phosphatase activity of the DNA kinase from rat liver.
Biochem Biophys Res Commun. 1982 Dec 31;109(4):1297-302. doi: 10.1016/0006-291x(82)91918-0.
4
DNA is linked to the rat liver DNA nicking-closing enzyme by a phosphodiester bond to tyrosine.DNA通过磷酸二酯键与大鼠肝脏DNA切口封闭酶的酪氨酸相连。
J Biol Chem. 1981 May 25;256(10):4805-9.
5
3'-Phosphatase activity in T4 polynucleotide kinase.T4多核苷酸激酶中的3'-磷酸酶活性。
Biochemistry. 1977 Nov 15;16(23):5120-6. doi: 10.1021/bi00642a027.

染色质3'-磷酸酶/5'-羟基激酶无法跨越DNA链上的切口将磷酸从3'端转移至5'端。

Chromatin 3'-phosphatase/5'-OH kinase cannot transfer phosphate from 3' to 5' across a strand nick in DNA.

作者信息

Habraken Y, Verly W G

出版信息

Nucleic Acids Res. 1986 Oct 24;14(20):8103-10. doi: 10.1093/nar/14.20.8103.

DOI:10.1093/nar/14.20.8103
PMID:3022244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311837/
Abstract

Rat liver chromatin contains a 3'-phosphatase/5'-OH kinase which may be involved in the repair of DNA strand breaks limited by 3'-phosphate/5'-OH ends. In order to determine whether the phosphate group can be transferred directly from the 3' to the 5' position, a polynucleotide duplex was synthesized between poly (dA) and oligo (dT) segments which had 3'-[32P]phosphate and 5'-OH ends. The oligo (dT) segments were separated by simple nicks as shown by the ability of T4 DNA ligase to seal the nick after the 3'-phosphate was removed by a phosphatase and the 5' end was phosphorylated with a kinase. The chromatin 3'-phosphatase/5'-OH kinase was unable to transfer phosphate directly from the 3' to the 5' end of the oligo (dT) segments in the original duplex; ATP was needed to phosphorylate the 5'-OH end. It is concluded that the chromatin 3'-phosphatase/5'-OH kinase is unable to convert a 3'-phosphate/5'-OH nick which cannot be repaired by DNA ligase directly into a 3'-OH/5'-phosphate nick which can be repaired by DNA ligase; the chromatin enzyme rather acts in two steps: hydrolysis of the 3'-phosphate followed by ATP-mediated phosphorylation of the 5'-OH end.

摘要

大鼠肝脏染色质含有一种3'-磷酸酶/5'-羟基激酶，它可能参与由3'-磷酸/5'-羟基末端限制的DNA链断裂的修复。为了确定磷酸基团是否可以直接从3'位转移到5'位，在聚（dA）和具有3'-[32P]磷酸和5'-羟基末端的寡聚（dT）片段之间合成了多核苷酸双链体。寡聚（dT）片段通过简单的切口分开，这可通过T4 DNA连接酶在磷酸酶去除3'-磷酸且5'末端用激酶磷酸化后封闭切口的能力来证明。染色质3'-磷酸酶/5'-羟基激酶无法将磷酸直接从原始双链体中寡聚（dT）片段的3'位转移到5'末端；需要ATP来磷酸化5'-羟基末端。结论是，染色质3'-磷酸酶/5'-羟基激酶无法将DNA连接酶无法直接修复的3'-磷酸/5'-羟基切口直接转化为DNA连接酶可以修复的3'-羟基/5'-磷酸切口；染色质酶而是分两步起作用：3'-磷酸的水解，随后是ATP介导的5'-羟基末端的磷酸化。