Moudi Bita, Heidari Zahra, Mahmoudzadeh-Sagheb Hamidreza
Zahedan University of Medical Sciences, Infectious Diseases and Tropical Medicine Research Center, and Department of Histology.
Eur J Histochem. 2018 Sep 18;62(3):2950. doi: 10.4081/ejh.2018.2950.
Hepatocellular carcinoma is one of the main consequences of liver chronic disease. Hepatocellular carcinoma-related changes may be seen in patients with chronic hepatitis B. The aim of the current study was to quantitate liver tissue elements by stereological technique in patients with hepatitis B-related cancer and compare the results with control and only hepatitis B group. Needle liver biopsies from 40 patients with only chronic hepatitis B infection, from 41 patients with only early hepatocellular carcinoma, from 40 patients with early hepatitis B-related cancer and 30 healthy subjects (control group) were analyzed by stereological method using systematic uniform random sampling method. Haematoxylin and eosin stained sections were used. The numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue were assessed. Quantitative analysis of liver samples indicated that there were statistically significant differences in the numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue between control and hepatitis B-related cancer and hepatitis B groups. Quantitative, stereological technique is simple and reliable for evaluating HCC in chronic hepatitis B. It is useful for assessing the liver tissue parameters. Stereology is recommended for the diagnosis of people prone to cancer in patients with chronic hepatitis B.
肝细胞癌是肝脏慢性疾病的主要后果之一。慢性乙型肝炎患者可能会出现与肝细胞癌相关的变化。本研究的目的是通过体视学技术对乙型肝炎相关癌症患者的肝组织成分进行定量分析，并将结果与对照组和单纯乙型肝炎组进行比较。采用系统均匀随机抽样法，对40例单纯慢性乙型肝炎感染患者、41例单纯早期肝细胞癌患者、40例早期乙型肝炎相关癌症患者和30名健康受试者（对照组）的肝脏穿刺活检组织进行体视学分析。使用苏木精和伊红染色切片。评估肝细胞的数量密度、肝细胞体积、库普弗细胞的数量密度、门管区结缔组织的体积密度以及结缔组织的体积密度。肝脏样本的定量分析表明，对照组与乙型肝炎相关癌症组和乙型肝炎组在肝细胞数量密度、肝细胞体积、库普弗细胞数量密度、门管区结缔组织体积密度以及结缔组织体积密度方面存在统计学显著差异。定量体视学技术在评估慢性乙型肝炎中的肝细胞癌方面简单可靠。它有助于评估肝组织参数。推荐使用体视学方法对慢性乙型肝炎中易患癌症的人群进行诊断。
