高纯度α-1-抗胰蛋白酶的分离与鉴定

Isolation and characterization of highly purified alpha-1-antitrypsin.

作者信息

Plancot M T, Delacourte A, Han K K, Dautrevaux M, Biserte G

出版信息

Int J Pept Protein Res. 1977;10(2):113-9. doi: 10.1111/j.1399-3011.1977.tb02784.x.

DOI:10.1111/j.1399-3011.1977.tb02784.x

Abstract

Highly purified human alpha-1-antitrypsin (phenotype MM) was obtained by an original method of preparative electrophoresis. The criteria of homogeneity were assured by one arc in crossed immunoelectrophoresis and one band on polyacrylamide gel. A unique N-terminal amino acid (pyroglutamic acid) and a unique C-terminal residue (lysine) were identified. Determined by gel electrophoresis, its molecular weight was 47,000 daltons.

摘要

通过一种制备电泳的原始方法获得了高度纯化的人α-1-抗胰蛋白酶（MM表型）。通过交叉免疫电泳中的一条弧线和聚丙烯酰胺凝胶上的一条带确保了均一性标准。鉴定出一个独特的N端氨基酸（焦谷氨酸）和一个独特的C端残基（赖氨酸）。通过凝胶电泳测定，其分子量为47,000道尔顿。