Tomita Y, Fukushima M, Tagami H
Tohoku J Exp Med. 1986 Aug;149(4):451-2. doi: 10.1620/tjem.149.451.
An increase in the amount of tyrosinase was demonstrated in the cultured human melanocyte after 6-day culturing with cholecalciferol (vitamin D3) by increased intensity of the immunofluorescent staining using monoclonal antibody against tyrosinase. Furthermore, the melanocytes became more dendritic as noted in those in the skin after the irradiation of ultraviolet. However, 7-dehydrocholesterol (pro-vitamin D3) or 1 alpha, 25-dihydroxy-vitamin D3 (activated vitamin D3) did not induce any such effect on the cultured human melanocytes. Since cholecalciferol is known to be photo-chemically converted by the ultraviolet irradiation from pro-vitamin D3 produced in the skin, the so-far-unknown mechanism of human skin pigmentation after the ultraviolet irradiation may be partly explained by this stimulating effect of vitamin D3 on the melanocytes.
用抗酪氨酸酶单克隆抗体进行免疫荧光染色，结果显示，经胆钙化醇（维生素D3）培养6天后的人培养黑素细胞中，酪氨酸酶含量增加。此外，黑素细胞变得更具树突状，这与紫外线照射后的皮肤黑素细胞情况相似。然而，7-脱氢胆固醇（维生素D3原）或1α,25-二羟基维生素D3（活性维生素D3）对人培养黑素细胞未产生任何此类作用。由于已知胆钙化醇可通过紫外线照射由皮肤中产生的维生素D3原进行光化学转化，因此，紫外线照射后人体皮肤色素沉着这一迄今未知的机制，可能部分是由维生素D3对黑素细胞的这种刺激作用所解释的。
