Bojnordi M N, Haratizadeh S, Darabi S, Hamidabadi H G
Bratisl Lek Listy. 2018;119(9):550-553. doi: 10.4149/BLL_2018_099.
Human dental pulp stem cells (hDPSCs) are multipotent stem cells providing an autologous noninvasive cell source. The study evaluates the neurogenic potential of hDPSCs using neural growth factor inducers and neurosphere technique.
The hDPSCs were differentiated into neurons using neural induction medium containing retinoic acid (RA). Neuroprogenitor cells were evaluated for nestin and NF68 using immunocytochemistry. The mature neuron markers, MAP‑2 and β-tubulin, were investigated at the end stage of induction phase.
The neuroprogenitor differentiation was confirmed by immunostaining for nestin and NF68 markers. The differentiated neurons were positive for specific neuron markers, namely for MAP‑2 and β-tubulin. The results indicated that the neural differentiation medium and neurosphere technique improve the generation of neuroprogenitor cells as well as mature neurons via exhibiting specific neural markers, namely nestin, NF68, MAP‑2 and β-tubulin.
Our findings highlight the differentiation capacity of hDPSCs via neurosphere technique in the presence of neural inducers for mesenchymal stem cells. It is suggested that the neural differentiation potential of hDPSCs can be exploited as a source of stem cells for therapy of neurodegenerative diseases (Fig. 5, Ref. 20).
人牙髓干细胞（hDPSCs）是多能干细胞，可提供自体非侵入性细胞来源。本研究使用神经生长因子诱导剂和神经球技术评估hDPSCs的神经分化潜能。
使用含有视黄酸（RA）的神经诱导培养基将hDPSCs分化为神经元。使用免疫细胞化学法评估神经祖细胞的巢蛋白和神经丝蛋白68（NF68）。在诱导阶段末期研究成熟神经元标志物微管相关蛋白2（MAP-2）和β-微管蛋白。
通过巢蛋白和NF68标志物的免疫染色证实了神经祖细胞的分化。分化的神经元对特定神经元标志物呈阳性，即MAP-2和β-微管蛋白。结果表明，神经分化培养基和神经球技术通过展示特定神经标志物，即巢蛋白、NF68、MAP-2和β-微管蛋白，改善了神经祖细胞以及成熟神经元的生成。
我们的研究结果突出了在存在间充质干细胞神经诱导剂的情况下，hDPSCs通过神经球技术的分化能力。建议将hDPSCs的神经分化潜能用作神经退行性疾病治疗的干细胞来源（图5，参考文献20）。
