Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.
Department of Pathology, Affiliated Hospital of Logistic University of PAP, Tianjin, China.
PLoS One. 2018 Sep 18;13(9):e0204051. doi: 10.1371/journal.pone.0204051. eCollection 2018.
Traumatic brain injury (TBI) is associated with trauma-related death. In this study, we evaluated differences in the expression of plasma microRNAs (miRNAs) in patients with different degrees of TBI, and explored the potential of miRNAs for use as diagnostic TBI biomarkers. The miRNA microarray results showed upregulation of 65, 33, and 16 miRNAs and downregulation of 29, 27, and 6 miRNAs in patients with mild, moderate, and severe TBI, respectively, compared with healthy controls. Thirteen miRNAs (seven upregulated and six downregulated) were found to be present in all TBI groups. Seven upregulated miRNAs were selected for validation in an enlarged cohort of samples and showed good diagnostic accuracy. The expression levels of miR-3195 and miR-328-5p were higher in the severe TBI group than in the mild and moderate TBI groups. In summary, our study demonstrates different expression profiles in plasma miRNAs among patients with mild to severe TBI. A subset of seven miRNAs can be used for diagnosis of TBI. Moreover, miR-3195 and miR-328-5p may be utilized during diagnosis to distinguish mild and moderate TBI from severe TBI.
创伤性脑损伤 (TBI) 与创伤相关的死亡有关。在这项研究中，我们评估了不同程度 TBI 患者血浆 microRNAs (miRNAs) 表达的差异，并探讨了 miRNAs 作为 TBI 诊断生物标志物的潜力。miRNA 微阵列结果显示，与健康对照组相比，轻度、中度和重度 TBI 患者的血浆中分别有 65、33 和 16 个 miRNAs 上调，29、27 和 6 个 miRNAs 下调。在所有 TBI 组中均发现有 13 个 miRNAs（7 个上调和 6 个下调）存在。选择了七个上调的 miRNAs 在更大的样本队列中进行验证，显示出良好的诊断准确性。miR-3195 和 miR-328-5p 在重度 TBI 组中的表达水平高于轻度和中度 TBI 组。总之，我们的研究表明，轻度至重度 TBI 患者血浆 miRNAs 的表达谱存在差异。一组七个 miRNAs 可用于 TBI 的诊断。此外，miR-3195 和 miR-328-5p 可能在诊断中用于区分轻度和中度 TBI 与重度 TBI。
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