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SacI、PvuII和EcoRI核酸内切酶的反应。

The reactions of SacI, PvuII and EcoRI endonucleases.

作者信息

Nasri M, Thomas D

出版信息

Biomed Biochim Acta. 1986;45(8):997-1005.

PMID:3022712
Abstract

A new approach to the mechanism study of DNA restriction is suggested. It is based on enzymatic hydrolysis of DNA in the presence of organic solvent. We found that in the presence of DMSO (10% v/v), the superhelical cleavage of pBR322 DNA by restriction endonucleases SacI and EcoRI proceeds with extensive accumulation of an intermediate, the single-nicked circular DNA, while with PvuII endonuclease the superhelical form is converted directly to the linear form.

摘要

提出了一种研究DNA限制机制的新方法。它基于在有机溶剂存在下对DNA进行酶促水解。我们发现，在二甲基亚砜（DMSO，体积分数10%）存在的情况下，限制性内切酶SacI和EcoRI对pBR322 DNA的超螺旋切割过程中，会大量积累一种中间体，即单切口环状DNA，而对于PvuII内切酶，超螺旋形式会直接转化为线性形式。

相似文献

1
The reactions of SacI, PvuII and EcoRI endonucleases.SacI、PvuII和EcoRI核酸内切酶的反应。
Biomed Biochim Acta. 1986;45(8):997-1005.
2
[Reaction of endonuclease EcoRI with DNA of the plasmid ColEI].
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Simultaneous binding of three recognition sites is necessary for a concerted plasmid DNA cleavage by EcoRII restriction endonuclease.为了使EcoRII限制性内切核酸酶协同切割质粒DNA，三个识别位点的同时结合是必要的。
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[Substrate specificity of restriction endonuclease Eco781].[限制性内切酶Eco781的底物特异性]
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Alteration of the specificity of PvuII restriction endonuclease.PvuII限制性内切酶特异性的改变。
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引用本文的文献

1
Changes in solvation during DNA binding and cleavage are critical to altered specificity of the EcoRI endonuclease.DNA结合和切割过程中溶剂化作用的变化对于EcoRI核酸内切酶特异性的改变至关重要。
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2186-91. doi: 10.1073/pnas.95.5.2186.
2
Heterogeneity in molecular recognition by restriction endonucleases: osmotic and hydrostatic pressure effects on BamHI, Pvu II, and EcoRV specificity.限制性内切核酸酶分子识别中的异质性：渗透压和流体静压力对BamHI、Pvu II和EcoRV特异性的影响
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3444-8. doi: 10.1073/pnas.92.8.3444.