Chemical Engineering Department , Federal University of Paraná , Francisco H. dos Santos Street , 81531-980 Curitiba , Paraná , Brazil.
J Agric Food Chem. 2018 Oct 10;66(40):10543-10551. doi: 10.1021/acs.jafc.8b03142. Epub 2018 Sep 28.
Phytate decreases mineral and protein availability and influences protein properties, such as solubility and stability. The binding constants and turbidity data can help with the understanding of the influence of phytate and divalent salts on protein behavior. Ternary complexes formed between bovine serum albumin, sodium phytate, and divalent salts were investigated by isothermal titration calorimetry, turbidity, and in vitro protein digestibility. Results showed a positive entropy change and a negative and small enthalpy change as a result of electrostatic binding forces and ternary and binary complex precipitation. The interaction was favored for the systems containing calcium and manganese, whereas those containing magnesium showed a low heat of interaction. Despite the high protein digestibility, the stability of divalent phytates in a wide pH range may decrease mineral bioavailability. These results can provide important insights for the study of mineral bioavailability and diverse processes that involve protein and minerals in several areas of knowledge.
植酸会降低矿物质和蛋白质的生物利用率，并影响蛋白质的特性，如溶解度和稳定性。结合常数和浊度数据可以帮助理解植酸和二价盐对蛋白质行为的影响。通过等温滴定量热法、浊度和体外蛋白质消化率研究了牛血清白蛋白、植酸钠和二价盐之间形成的三元络合物。结果表明，静电结合力和三元及二元络合物沉淀导致正熵变和小的负焓变。对于含有钙和锰的体系，这种相互作用是有利的，而含有镁的体系则表现出较低的相互作用热。尽管蛋白质消化率很高，但在较宽的 pH 范围内，二价植酸盐的稳定性可能会降低矿物质的生物利用率。这些结果可为研究矿物质生物利用率以及涉及知识领域中蛋白质和矿物质的多种过程提供重要的见解。
