• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

血液透析相关的单核细胞表型特征变化。

Hemodialysis-related changes in phenotypical features of monocytes.

机构信息

Clinic of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke-University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.

Division of Nephrology and Hypertension, 1st Department of Internal Medicine, AHEPA Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.

出版信息

Sci Rep. 2018 Sep 18;8(1):13964. doi: 10.1038/s41598-018-31889-2.

DOI:10.1038/s41598-018-31889-2
PMID:30228352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6143543/
Abstract

Hemodialysis (HD) patients exhibit chronic inflammation and leukocyte activation. We investigated the surface-marker profile of monocytes by flow cytometry to assess the chronic effect of uremia and the acute effect of dialysis on their phenotypical and functional features in 16 healthy controls (CON) and 15 HD patients before and after a polysulfone-based dialysis session. Median fluorescence intensities were analyzed indicating expression of CD14, CD16, integrins (CD11b, CD18), chemokine receptors (CCR2, CX3CR1), scavenger receptors (CD36, CD163) and Toll-like receptor-2 (TLR2). Before and after dialysis, HD patients harbour 0.9-fold less CD14CD16 (Mo1), 1.8-fold more CD14CD16 (Mo2) and CD14CD16 (Mo3) monocytes than CON. HD patients' Mo1 showed elevated expression of CD11b (1.7-fold), CD18 (1.2-fold) and CD36 (2.1-fold), whereas CD163 expression was reduced in Mo1 and Mo2 (0.6-fold) compared to CON. These markers remained unaffected by dialysis. CX3CR1 expression on Mo2 and Mo3 was lower in HD patients before (0.8-fold) and further diminished after dialysis (0.6-fold). Stimulation of monocytes resulted in diminished responses in HD patients compared to CON. In conclusion, a systematic analysis of the expression of particular surface markers on distinct monocyte subsets may help to distinguish between uremia and/or dialysis induced effects and to evaluate the functionality of monocytes and biocompatibility of HD.

摘要

血液透析 (HD) 患者表现出慢性炎症和白细胞激活。我们通过流式细胞术研究了单核细胞的表面标志物谱，以评估尿毒症的慢性影响以及透析对其表型和功能特征的急性影响，共纳入 16 名健康对照 (CON) 和 15 名 HD 患者，分别在透析前后进行检测。分析了中荧光强度，以表明 CD14、CD16、整合素 (CD11b、CD18)、趋化因子受体 (CCR2、CX3CR1)、清道夫受体 (CD36、CD163) 和 Toll 样受体-2 (TLR2) 的表达。在透析前后，HD 患者的 Mo1 中 CD14CD16 细胞减少了 0.9 倍，Mo2 和 Mo3 中 CD14CD16 细胞增加了 1.8 倍，比 CON 多。HD 患者的 Mo1 中 CD11b (1.7 倍)、CD18 (1.2 倍) 和 CD36 (2.1 倍) 的表达增加，而 Mo1 和 Mo2 中的 CD163 表达减少 (0.6 倍)。这些标志物不受透析影响。Mo2 和 Mo3 上的 CX3CR1 表达在透析前 (0.8 倍) 和透析后 (0.6 倍) 均降低。与 CON 相比，HD 患者的单核细胞刺激反应减弱。总之，对特定单核细胞亚群上特定表面标志物的表达进行系统分析，可能有助于区分尿毒症和/或透析引起的影响，并评估单核细胞的功能和血液透析的生物相容性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/558ff1347d90/41598_2018_31889_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/1fa0dd682806/41598_2018_31889_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/36d5d9c048ea/41598_2018_31889_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/55b484dd28a4/41598_2018_31889_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/1e6465f0fa75/41598_2018_31889_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/b3c95a28329a/41598_2018_31889_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/558ff1347d90/41598_2018_31889_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/1fa0dd682806/41598_2018_31889_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/36d5d9c048ea/41598_2018_31889_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/55b484dd28a4/41598_2018_31889_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/1e6465f0fa75/41598_2018_31889_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/b3c95a28329a/41598_2018_31889_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dcd/6143543/558ff1347d90/41598_2018_31889_Fig6_HTML.jpg

相似文献

1
Hemodialysis-related changes in phenotypical features of monocytes.血液透析相关的单核细胞表型特征变化。
Sci Rep. 2018 Sep 18;8(1):13964. doi: 10.1038/s41598-018-31889-2.
2
Contribution of the uremic milieu to an increased pro-inflammatory monocytic phenotype in chronic kidney disease.尿毒症环境对慢性肾脏病中促炎单核细胞表型增加的贡献。
Sci Rep. 2019 Jul 15;9(1):10236. doi: 10.1038/s41598-019-46724-5.
3
Apoptotic markers on lymphocytes and monocytes are unchanged during single hemodialysis sessions using either regenerated cellulose or polysulfone membranes.在使用再生纤维素或聚砜膜进行单次血液透析治疗期间，淋巴细胞和单核细胞上的凋亡标志物未发生变化。
Clin Nephrol. 2005 Sep;64(3):198-204. doi: 10.5414/cnp64198.
4
Expression of Fc gamma receptor III (CD16) on monocytes during hemodialysis in patients with chronic renal failure.慢性肾衰竭患者血液透析期间单核细胞上Fcγ受体III（CD16）的表达
Nephron. 2002 Jan;90(1):64-71. doi: 10.1159/000046316.
5
Haemodialysis monocytopenia: differential sequestration kinetics of CD14+CD16+ and CD14++ blood monocyte subsets.血液透析导致的单核细胞减少：CD14+CD16+和CD14++血液单核细胞亚群的差异性隔离动力学
Clin Exp Immunol. 2001 Jan;123(1):49-55. doi: 10.1046/j.1365-2249.2001.01436.x.
6
Biocompatibility study based on differential sequestration kinetics of CD14+CD16+ blood monocyte subsets with different dialyzers.基于不同透析器对CD14+CD16+血液单核细胞亚群的差异隔离动力学的生物相容性研究
Ren Fail. 2006;28(6):493-9. doi: 10.1080/08860220600781336.
7
Strong depletion of CD14(+)CD16(+) monocytes during haemodialysis treatment.血液透析治疗期间CD14(+)CD16(+)单核细胞严重耗竭。
Nephrol Dial Transplant. 2001 Jul;16(7):1402-8. doi: 10.1093/ndt/16.7.1402.
8
Monocyte cell-surface CD14 expression and soluble CD14 antigen in hemodialysis: evidence for chronic exposure to LPS.血液透析中单核细胞表面CD14表达及可溶性CD14抗原：长期暴露于脂多糖的证据
Kidney Int. 1995 Nov;48(5):1469-76. doi: 10.1038/ki.1995.436.
9
Heterogeneity of human monocytes: an optimized four-color flow cytometry protocol for analysis of monocyte subsets.人源单核细胞的异质性：一种优化的四色流式细胞术分析单核细胞亚群的方案。
J Cardiovasc Transl Res. 2011 Apr;4(2):211-9. doi: 10.1007/s12265-011-9256-4. Epub 2011 Feb 10.
10
Microinflammation in hemodialysis is related to a preactivated subset of monocytes.血液透析中的微炎症与单核细胞的一个预激活亚群有关。
Hemodial Int. 2006 Jan;10 Suppl 1:S24-7. doi: 10.1111/j.1542-4758.2006.01186.x.

引用本文的文献

1
Leukocyte Dysregulation and Biochemical Alterations in End-Stage Kidney Disease Patients Under Hemodialysis.血液透析终末期肾病患者的白细胞调节异常及生化改变
Diseases. 2025 Mar 21;13(4):90. doi: 10.3390/diseases13040090.
2
The Complex Immunological Alterations in Patients with Type 2 Diabetes Mellitus on Hemodialysis.接受血液透析的2型糖尿病患者的复杂免疫改变
J Clin Med. 2024 Jun 25;13(13):3687. doi: 10.3390/jcm13133687.
3
Monocytes as Targets for Immunomodulation by Regional Citrate Anticoagulation.单核细胞作为局部枸橼酸抗凝免疫调节的靶点。

本文引用的文献

1
CX3CR1 mononuclear phagocytes control immunity to intestinal fungi.CX3CR1单核吞噬细胞控制对肠道真菌的免疫。
Science. 2018 Jan 12;359(6372):232-236. doi: 10.1126/science.aao1503.
2
TLR4-dependent internalization of CX3CR1 aggravates sepsis-induced immunoparalysis.TLR4 依赖的 CX3CR1 内化加重脓毒症诱导的免疫麻痹。
Am J Transl Res. 2016 Dec 15;8(12):5696-5705. eCollection 2016.
3
Decreased CX3CR1 messenger RNA expression is an independent molecular biomarker of early and late mortality in critically ill patients.CX3CR1信使核糖核酸表达降低是危重症患者早期和晚期死亡的独立分子生物标志物。
Int J Mol Sci. 2024 Mar 1;25(5):2900. doi: 10.3390/ijms25052900.
4
Neutrophil extracellular trap is an important connection between hemodialysis and acute myocardial infarction.中性粒细胞胞外诱捕网是血液透析与急性心肌梗死之间的重要联系。
Ren Fail. 2023 Dec;45(1):2216307. doi: 10.1080/0886022X.2023.2216307.
5
Differences in the Profile of Circulating Immune Cell Subsets in Males with Type 2 Cardiorenal Syndrome versus CKD Patients without Established Cardiovascular Disease.2型心肾综合征男性患者与无确诊心血管疾病的慢性肾脏病患者循环免疫细胞亚群谱的差异。
Biomedicines. 2023 Mar 27;11(4):1029. doi: 10.3390/biomedicines11041029.
6
Hemodialysis-Associated Immune Dysregulation in SARS-CoV-2-Infected End-Stage Renal Disease Patients.COVID-19 相关免疫失调与血液透析患者 **解析**：原文中“SARS-CoV-2”为“严重急性呼吸综合征冠状病毒 2”的缩写，简称“新冠病毒”，在译文保留英文缩写。“End-Stage Renal Disease”是“终末期肾病”的英文缩写，在译文也保留了英文缩写。
Int J Mol Sci. 2023 Jan 15;24(2):1712. doi: 10.3390/ijms24021712.
7
Immune System Dysfunction and Inflammation in Hemodialysis Patients: Two Sides of the Same Coin.血液透析患者的免疫系统功能障碍与炎症：同一硬币的两面
J Clin Med. 2022 Jun 28;11(13):3759. doi: 10.3390/jcm11133759.
8
Single-Cell RNA and ATAC Sequencing Reveal Hemodialysis-Related Immune Dysregulation of Circulating Immune Cell Subpopulations.单细胞 RNA 和 ATAC 测序揭示了血液透析相关的循环免疫细胞亚群免疫失调。
Front Immunol. 2022 May 26;13:878226. doi: 10.3389/fimmu.2022.878226. eCollection 2022.
9
Oxidative Stress in Patients with Advanced CKD and Renal Replacement Therapy: The Key Role of Peripheral Blood Leukocytes.晚期慢性肾脏病患者及肾脏替代治疗中的氧化应激：外周血白细胞的关键作用
Antioxidants (Basel). 2021 Jul 20;10(7):1155. doi: 10.3390/antiox10071155.
10
Regulation and function of CX3CR1 and its ligand CX3CL1 in kidney disease.CX3CR1 及其配体 CX3CL1 在肾脏疾病中的调节和功能。
Cell Tissue Res. 2021 Aug;385(2):335-344. doi: 10.1007/s00441-021-03473-0. Epub 2021 May 19.
Crit Care. 2016 Jun 30;20(1):204. doi: 10.1186/s13054-016-1362-x.
4
Monocyte CD163 is altered in association with diabetic complications: possible protective role.单核细胞 CD163 与糖尿病并发症相关改变：可能具有保护作用。
J Leukoc Biol. 2016 Dec;100(6):1375-1383. doi: 10.1189/jlb.3A1015-461RR. Epub 2016 Jun 27.
5
Perspectives for Monocyte/Macrophage-Based Diagnostics of Chronic Inflammation.基于单核细胞/巨噬细胞的慢性炎症诊断前景
Transfus Med Hemother. 2016 Mar;43(2):66-77. doi: 10.1159/000444943. Epub 2016 Mar 15.
6
Evaluation of the Biocompatibility of Dialysis Membranes.透析膜生物相容性的评估
Blood Purif. 2015;40(4):293-7. doi: 10.1159/000441576. Epub 2015 Nov 17.
7
Non-Classical monocytes display inflammatory features: Validation in Sepsis and Systemic Lupus Erythematous.非经典单核细胞表现出炎症特征：在脓毒症和系统性红斑狼疮中的验证
Sci Rep. 2015 Sep 11;5:13886. doi: 10.1038/srep13886.
8
Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation.终末期肾病中单核细胞衍生树突状细胞的分化倾向于加速成熟。
Adv Clin Exp Med. 2015 Mar-Apr;24(2):257-66. doi: 10.17219/acem/40463.
9
Transcriptome analysis in patients with chronic kidney disease on hemodialysis disclosing a key role for CD16+CX3CR1+ monocytes.血液透析慢性肾病患者的转录组分析揭示了CD16+CX3CR1+单核细胞的关键作用。
PLoS One. 2015 Apr 1;10(4):e0121750. doi: 10.1371/journal.pone.0121750. eCollection 2015.
10
Update on inflammation in chronic kidney disease.慢性肾脏病炎症研究进展
Blood Purif. 2015;39(1-3):84-92. doi: 10.1159/000368940. Epub 2015 Jan 20.