Stephens E B, Compans R W
Cell. 1986 Dec 26;47(6):1053-9. doi: 10.1016/0092-8674(86)90820-2.
Vaccinia virus recombinants were generated which express the intact gp70/p15E of Friend mink cell focus inducing virus (F-MCFV) or truncated forms of the glycoprotein that lack the transmembrane and cytoplasmic domains. The transport of the intact and truncated envelope glycoproteins to apical or basolateral surfaces was studied in the polarized epithelial MDCK cell line. Infection of MDCK cells with the recombinant expressing the intact F-MCFV envelope glycoprotein resulted in transport exclusively to the basolateral surfaces, whereas the recombinant expressing the truncated glycoprotein was found to be secreted from both the apical and basolateral surfaces. Thus removal of the transmembrane and cytoplasmic domains of the p15E protein results in a loss of directional transport to the basolateral membrane of polarized epithelial cells.
构建了痘苗病毒重组体，其表达弗氏水貂细胞灶诱导病毒（F-MCFV）完整的gp70/p15E或缺乏跨膜和胞质结构域的糖蛋白截短形式。在极化上皮MDCK细胞系中研究了完整和截短包膜糖蛋白向顶端或基底外侧表面的转运。用表达完整F-MCFV包膜糖蛋白的重组体感染MDCK细胞，结果显示其仅转运至基底外侧表面，而表达截短糖蛋白的重组体则从顶端和基底外侧表面均分泌。因此，去除p15E蛋白的跨膜和胞质结构域会导致向极化上皮细胞基底外侧膜的定向转运丧失。
