Bender A, Sprague G F
Cell. 1986 Dec 26;47(6):929-37. doi: 10.1016/0092-8674(86)90808-1.
We show that in yeast the cell type specificity of pheromone response is determined solely by the species of receptor that a cell synthesizes. The two receptor-pheromone interactions are functionally interchangeable and involve the creation of a common intracellular signal. In particular, we find that provision of a-factor receptor or alpha-factor receptor in mat alpha 1 mutants, which normally do not express either receptor or any other a- or alpha-specific products, allows response to the appropriate pheromone. Moreover, provision of a-factor receptor in a cells lacking alpha-factor receptor restores mating competence to those cells. Finally, an aspect of pheromone response that is normally unique to a-factor action on alpha cells--increased transcription from the alpha-specific STE3 gene--can also be observed following alpha-factor treatment of pseudo-a cells (mat alpha 2 ste3 ste13), special mutants that respond to alpha-factor and also have an active STE3 promoter.
我们发现，在酵母中，信息素反应的细胞类型特异性完全由细胞合成的受体种类决定。两种受体 - 信息素相互作用在功能上是可互换的，并且涉及共同细胞内信号的产生。具体而言，我们发现，在通常不表达任何一种受体或任何其他a - 或α - 特异性产物的matα1突变体中提供a - 因子受体或α - 因子受体，可使细胞对相应的信息素产生反应。此外，在缺乏α - 因子受体的a细胞中提供a - 因子受体可恢复这些细胞的交配能力。最后，在对α - 因子有反应且具有活性STE3启动子的特殊突变体——假a细胞（matα2 ste3 ste13）经α - 因子处理后，也能观察到信息素反应中通常是a - 因子作用于α细胞所特有的一个方面——α - 特异性STE3基因转录增加。
