Gartia Janeka, Barnwal Ravi Pratap, Anangi Raveendra, Giri Ashok R, King Glenn, Chary Kandala V R
Center for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Gopanpally, Hyderabad, 500075, India.
Department of Biophysics, Panjab University, Chandigarh, 160014, India.
Biomol NMR Assign. 2019 Apr;13(1):31-35. doi: 10.1007/s12104-018-9846-z. Epub 2018 Sep 18.
Helicoverpa species are polyphagous pests, with the larval stages causing major damage to economically valuable crops such as cotton, tomato, corn, sorghum, peas, sunflower, wheat and other pulses. Over the years, Helicoverpa armigera has developed resistance to most classes of chemical insecticides, and consequently it is now largely controlled on cotton plants via the use of Bt transgenic crops that express insecticidal Cry toxins which in-turn expedited resistance development in a number of pest species including H. armigera. In a hope to provide other eco-friendly alternatives solutions to counter the effect of the pest, people have identified a number of protease inhibitors (PIs) from the domesticated capsicum species Capsicum annuum, several of which potently inhibited H. armigera gut proteases and impeded growth of H. armigera larva. With a view to explore and enhance the specific nature or properties of these PIs on the mechanism of inhibition, structural and functional characterization of these PIs are inevitable. Towards this goal, we have carried out complete H, C and N resonance assignments of two of these PIs, identified as IRD7 and IRD12, using a suite of 2D and 3D multi-dimensional and multi-nuclear NMR experiments.
棉铃虫是多食性害虫，其幼虫阶段会对棉花、番茄、玉米、高粱、豌豆、向日葵、小麦和其他豆类等经济作物造成严重损害。多年来，棉铃虫已对大多数种类的化学杀虫剂产生抗性，因此目前主要通过种植表达杀虫Cry毒素的Bt转基因作物来控制棉铃虫，而这反过来又加速了包括棉铃虫在内的许多害虫的抗性发展。为了提供其他环保替代解决方案来应对这种害虫的影响，人们从驯化的辣椒品种辣椒中鉴定出了多种蛋白酶抑制剂（PIs），其中几种能有效抑制棉铃虫肠道蛋白酶并阻碍棉铃虫幼虫生长。为了探索并增强这些PIs在抑制机制方面的特异性或特性，对这些PIs进行结构和功能表征是必不可少的。为了实现这一目标，我们使用一系列二维和三维多维多核核磁共振实验，对其中两种被鉴定为IRD7和IRD12的PIs进行了完整的氢、碳和氮共振归属。
