Department of Respiratory Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(17):5554-5561. doi: 10.26355/eurrev_201809_15818.
To explore the role of miR-451a in the migration and invasion of non-small cell lung cancer (NSCLC) cells.
Quantitative Real time-polymerase chain reaction (qRT-PCR) and Western blot were performed to detect the levels of miR-451a and activating transcription factor 2 (ATF2) in NSCLC. Transwell assay was employed to analyze the migratory and invasive abilities in NSCLC cells. Dual-luciferase reporter assay was applied to confirm the binding condition of miR-451 and its target gene in NSCLC cells.
MiR-451a was downregulated in NSCLC tissues and lung cancer cell lines A549 and NCI-H460, while ATF2 was upregulated. The mRNA level of miR-451a was negatively correlated to ATF2. Additionally, miR-451a regulated cell migration and invasion through targeting ATF2. Furthermore, ATF2 could reverse the inhibitory migration and invasion of A549 cells induced by miR-451a.
MiR-451a inhibits the migratory and invasive abilities of NSCLC cells through ATF2 regulation. The newly identified miR-451a/ATF2 axis provides a novel insight into the pathogenesis ofNSCLC.
探讨微小 RNA-451a（miR-451a）在非小细胞肺癌（NSCLC）细胞迁移和侵袭中的作用。
采用实时定量聚合酶链反应（qRT-PCR）和 Western blot 检测 NSCLC 中 miR-451a 和激活转录因子 2（ATF2）的水平。采用 Transwell 实验分析 NSCLC 细胞的迁移和侵袭能力。双荧光素酶报告基因实验用于验证 miR-451 在 NSCLC 细胞中的靶基因结合情况。
miR-451a 在 NSCLC 组织和肺癌细胞系 A549、NCI-H460 中表达下调，而 ATF2 表达上调。miR-451a 的 mRNA 水平与 ATF2 呈负相关。此外，miR-451a 通过靶向 ATF2 调节细胞迁移和侵袭。此外，ATF2 可逆转 miR-451a 诱导的 A549 细胞迁移和侵袭的抑制作用。
miR-451a 通过 ATF2 调节抑制 NSCLC 细胞的迁移和侵袭能力。新鉴定的 miR-451a/ATF2 轴为 NSCLC 的发病机制提供了新的见解。
