Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(17):5614-5619. doi: 10.26355/eurrev_201809_15826.
The pathogenesis of vascular dementia (VD) is not fully elucidated. Th1/Th2 balance may change in VD, leading to numerous inflammatory cytokines secretion. Interleukin-10 (IL-10) is an immune suppressor, while its function in VD and correlation with Th1/Th2 balance are still unclear.
The healthy male rats were randomly divided into three groups, including sham group, model group, and IL-10 group. Th1 and Th2 cytokines IL-2, IL-4, IL-6, and tumor necrosis factor-α (TNF-α) expressions in the serum were tested by enzyme-linked immunosorbent assay (ELISA). IL-10 expression in brain tissue and peripheral blood was detected by Real-Time PCR and ELISA. The correlation relationship between IL-10 and T helper cells 1/2 (Th1/Th2) cytokines was analyzed. Hippocampus cell apoptosis was determined by caspase 3 activity kit. Nuclear transcription factor 2 κB (NF-κB) expression was evaluated by Western blot.
IL-10 levels were decreased, caspase 3 activity was enhanced, NF-κB expression was declined, IL-2 and TNF-α secretion were up-regulated, while IL-4 and IL-6 secretion were reduced in hippocampus tissue and peripheral blood from VD model rat compared to sham group (p<0.05). IL-10 significantly attenuated caspase 3 activity, up-regulated NF-κB expression, reduced IL-2 and TNF-α secretion, and enhanced IL-4 and IL-6 secretion (p<0.05). IL-10 was negatively correlated with Th1 cytokines and positively correlated with Th2 cytokines (p<0.05).
IL-10 expression declined in VD and participated in regulating Th1/Th2 balance. IL-10 participated in VD incidence and development through regulating cell apoptosis and NF-κB expression.
血管性痴呆（VD）的发病机制尚未完全阐明。Th1/Th2 平衡可能在 VD 中发生改变，导致多种炎症细胞因子的分泌。白细胞介素-10（IL-10）是一种免疫抑制剂，但其在 VD 中的功能及其与 Th1/Th2 平衡的关系尚不清楚。
将健康雄性大鼠随机分为三组，包括假手术组、模型组和 IL-10 组。通过酶联免疫吸附试验（ELISA）检测血清中 Th1 和 Th2 细胞因子白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。通过实时 PCR 和 ELISA 检测脑组织和外周血中 IL-10 的表达。分析 IL-10 与辅助性 T 细胞 1/2（Th1/Th2）细胞因子的相关性。通过 caspase 3 活性试剂盒检测海马细胞凋亡。通过 Western blot 评估核转录因子 2 κB（NF-κB）的表达。
与假手术组相比，VD 模型大鼠海马组织和外周血中 IL-10 水平降低，caspase 3 活性增强，NF-κB 表达下调，IL-2 和 TNF-α分泌上调，IL-4 和 IL-6 分泌下调（p<0.05）。IL-10 显著减弱 caspase 3 活性，上调 NF-κB 表达，减少 IL-2 和 TNF-α分泌，增加 IL-4 和 IL-6 分泌（p<0.05）。IL-10 与 Th1 细胞因子呈负相关，与 Th2 细胞因子呈正相关（p<0.05）。
IL-10 在 VD 中表达下降，并参与调节 Th1/Th2 平衡。IL-10 通过调节细胞凋亡和 NF-κB 表达参与 VD 的发生和发展。
