Department of Cardiac Function, Xuzhou Central Hospital, Xuzhou, Jiangsu, China.
Department of Cardiology, Xuzhou Central Hospital, Xuzhou, Jiangsu, China.
Ann Clin Lab Sci. 2024 Jul;54(4):483-488.
Pregnancy induced hypertension (PIH) is a common disease in obstetrics. CD4+ T cells can be divided into Th1 and Th2 sub-populations. Imbalance between Th1 and Th2 directly affects body immune status and participates in PIH occurrence and progression. Whether IL-10 affects Th1/Th2 immune balance as a negative regulator of immune response in PIH remains unknown. The aim of the present study was to investigate the role of IL-10 in PIH.
A total of 52 PIH patients were recruited and divided into mild-moderate and severe PIH groups in parallel with 25 normal pregnant women as a control group. Real-time PCR was used to test mRNA levels of Th1 cytokines IL-2, tumor necrosis factor- (TNF-), and Th2 cytokines IL-4, IL-6, and IL-10. Enzyme linked immunosorbent assay (ELISA) tested serum levels of cytokines to analyze their correlation with disease progression.
Our results showed PIH patients had significantly elevated IL-2 and TNF- levels and decreased IL-4, IL-6, or IL-10 expressions compared with the control group (<0.05). With disease progression, IL-4, IL-6, and IL-10 expressions were further decreased while IL-2 and TNF- were increased (<0.05). Moreover, IL-10 was negatively correlated with Th1 cytokines IL-2 and TNF- while being positively correlated with Th2 cytokines IL-4 and IL-6. In addition, IL-10 was negatively correlated with PIH severity (<0.05).
IL-10 can affect Th1/Th2 immune balance and is associated with PIH severity, suggesting IL-10 might be a risk factor for PIH occurrence and progression.
妊娠高血压(PIH)是妇产科常见疾病。CD4+T 细胞可分为 Th1 和 Th2 亚群。Th1 和 Th2 的失衡直接影响机体免疫状态,并参与 PIH 的发生和进展。白细胞介素-10(IL-10)是否作为 PIH 免疫反应的负调节剂影响 Th1/Th2 免疫平衡尚不清楚。本研究旨在探讨 IL-10 在 PIH 中的作用。
共招募 52 例 PIH 患者,与 25 例正常妊娠妇女平行分为轻度-中度和重度 PIH 组。采用实时 PCR 检测 Th1 细胞因子白细胞介素-2(IL-2)、肿瘤坏死因子-(TNF-)和 Th2 细胞因子白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)的 mRNA 水平。酶联免疫吸附试验(ELISA)检测细胞因子血清水平,分析其与疾病进展的相关性。
结果显示,与对照组相比,PIH 患者的 IL-2 和 TNF-水平显著升高,IL-4、IL-6 和 IL-10 表达降低(<0.05)。随着病情进展,IL-4、IL-6 和 IL-10 表达进一步降低,而 IL-2 和 TNF-升高(<0.05)。此外,IL-10 与 Th1 细胞因子 IL-2 和 TNF-呈负相关,与 Th2 细胞因子 IL-4 和 IL-6 呈正相关。此外,IL-10 与 PIH 严重程度呈负相关(<0.05)。
IL-10 可影响 Th1/Th2 免疫平衡,与 PIH 严重程度相关,提示 IL-10 可能是 PIH 发生和进展的危险因素。