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白细胞介素-10 对 Th1/Th2 免疫平衡与妊娠高血压的相关性。

Correlation between Interleukin-10 Effects on Th1/Th2 Immune Balance and Pregnancy Induced Hypertension.

机构信息

Department of Cardiac Function, Xuzhou Central Hospital, Xuzhou, Jiangsu, China.

Department of Cardiology, Xuzhou Central Hospital, Xuzhou, Jiangsu, China.

出版信息

Ann Clin Lab Sci. 2024 Jul;54(4):483-488.

Abstract

OBJECTIVE

Pregnancy induced hypertension (PIH) is a common disease in obstetrics. CD4+ T cells can be divided into Th1 and Th2 sub-populations. Imbalance between Th1 and Th2 directly affects body immune status and participates in PIH occurrence and progression. Whether IL-10 affects Th1/Th2 immune balance as a negative regulator of immune response in PIH remains unknown. The aim of the present study was to investigate the role of IL-10 in PIH.

METHODS

A total of 52 PIH patients were recruited and divided into mild-moderate and severe PIH groups in parallel with 25 normal pregnant women as a control group. Real-time PCR was used to test mRNA levels of Th1 cytokines IL-2, tumor necrosis factor- (TNF-), and Th2 cytokines IL-4, IL-6, and IL-10. Enzyme linked immunosorbent assay (ELISA) tested serum levels of cytokines to analyze their correlation with disease progression.

RESULTS

Our results showed PIH patients had significantly elevated IL-2 and TNF- levels and decreased IL-4, IL-6, or IL-10 expressions compared with the control group (<0.05). With disease progression, IL-4, IL-6, and IL-10 expressions were further decreased while IL-2 and TNF- were increased (<0.05). Moreover, IL-10 was negatively correlated with Th1 cytokines IL-2 and TNF- while being positively correlated with Th2 cytokines IL-4 and IL-6. In addition, IL-10 was negatively correlated with PIH severity (<0.05).

CONCLUSION

IL-10 can affect Th1/Th2 immune balance and is associated with PIH severity, suggesting IL-10 might be a risk factor for PIH occurrence and progression.

摘要

目的

妊娠高血压(PIH)是妇产科常见疾病。CD4+T 细胞可分为 Th1 和 Th2 亚群。Th1 和 Th2 的失衡直接影响机体免疫状态,并参与 PIH 的发生和进展。白细胞介素-10(IL-10)是否作为 PIH 免疫反应的负调节剂影响 Th1/Th2 免疫平衡尚不清楚。本研究旨在探讨 IL-10 在 PIH 中的作用。

方法

共招募 52 例 PIH 患者,与 25 例正常妊娠妇女平行分为轻度-中度和重度 PIH 组。采用实时 PCR 检测 Th1 细胞因子白细胞介素-2(IL-2)、肿瘤坏死因子-(TNF-)和 Th2 细胞因子白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)的 mRNA 水平。酶联免疫吸附试验(ELISA)检测细胞因子血清水平,分析其与疾病进展的相关性。

结果

结果显示,与对照组相比,PIH 患者的 IL-2 和 TNF-水平显著升高,IL-4、IL-6 和 IL-10 表达降低(<0.05)。随着病情进展,IL-4、IL-6 和 IL-10 表达进一步降低,而 IL-2 和 TNF-升高(<0.05)。此外,IL-10 与 Th1 细胞因子 IL-2 和 TNF-呈负相关,与 Th2 细胞因子 IL-4 和 IL-6 呈正相关。此外,IL-10 与 PIH 严重程度呈负相关(<0.05)。

结论

IL-10 可影响 Th1/Th2 免疫平衡,与 PIH 严重程度相关,提示 IL-10 可能是 PIH 发生和进展的危险因素。

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