一种单克隆抗体与鸡胗结蛋白特异性钙蛋白酶催化亚基的交叉反应性。

Cross-reactivity of a monoclonal antibody to the catalytic subunit of chicken gizzard desmin-specific calpain.

作者信息

Aldstadt J H, Allen M J, Johnson P

出版信息

Comp Biochem Physiol B. 1986;85(2):355-63. doi: 10.1016/0305-0491(86)90012-x.

Abstract

The desmin-specific calpain I from chicken gizzard smooth muscle is a dimer of 83 and 35 kDalton subunits. A monoclonal antibody to the large subunit did not cross-react with chicken gizzard and hamster skeletal muscle calpain II, but it did recognize hamster skeletal muscle desmin-specific calpain I and the denatured calpain II from chicken gizzard smooth muscle. These results indicate that different desmin-specific calpains have similar large subunits which differ significantly from the large subunit of calpain II in the same tissue.

摘要

来自鸡肫平滑肌的结蛋白特异性钙蛋白酶Ⅰ是由83千道尔顿和35千道尔顿亚基组成的二聚体。针对大亚基的单克隆抗体与鸡肫和仓鼠骨骼肌的钙蛋白酶Ⅱ无交叉反应，但它能识别仓鼠骨骼肌结蛋白特异性钙蛋白酶Ⅰ以及鸡肫平滑肌的变性钙蛋白酶Ⅱ。这些结果表明，不同的结蛋白特异性钙蛋白酶具有相似的大亚基，这些大亚基与同一组织中钙蛋白酶Ⅱ的大亚基有显著差异。