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开源毛细管电泳。

Open source capillary electrophoresis.

机构信息

Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Brno, Czech Republic.

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.

出版信息

Electrophoresis. 2019 Jan;40(1):65-78. doi: 10.1002/elps.201800304. Epub 2018 Sep 27.

Abstract

Open source paradigm is becoming widely accepted in scientific communities and open source hardware is finding its steady place in chemistry research. In this review article, we provide the reader with the most up-to-date information on open source hardware and software resources enabling the construction and utilization of an "open source capillary electrophoresis instrument". While CE is still underused as a separation technique, it offers unique flexibility, low-cost, and high efficiency and is particularly suitable for open source instrumental development. We overview the major parts of CE instruments, such as high voltage power supplies, detectors, data acquisition systems, and CE software resources with emphasis on availability of the open source information on the web and in the scientific literature. This review is the first of its kind, revealing accessible blueprints of most parts from which a fully functional open source CE system can be built. By collecting the extensive information on open source capillary electrophoresis in this review article, the authors aim at facilitating the dissemination of knowledge on CE within and outside the scientific community, fosters innovation and inspire other researchers to improve the shared CE blueprints.

摘要

开源模式在科学界中越来越被广泛接受,开源硬件在化学研究中也找到了自己的稳定位置。在这篇综述文章中,我们为读者提供了最新的关于开源硬件和软件资源的信息,这些资源能够构建和利用“开源毛细管电泳仪”。尽管 CE 作为一种分离技术仍未得到充分应用,但它具有独特的灵活性、低成本和高效率,特别适合于开源仪器的开发。我们概述了 CE 仪器的主要部分,如高压电源、检测器、数据采集系统和 CE 软件资源,并重点介绍了网络和科学文献中开源信息的可用性。这是此类综述中的第一篇,揭示了大部分功能齐全的开源 CE 系统都可以从这些可用的蓝图中构建出来。通过在这篇综述文章中收集有关开源毛细管电泳的广泛信息,作者旨在促进科学界内外对 CE 的知识传播,鼓励创新,并激发其他研究人员改进共享的 CE 蓝图。

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