Aguilera G
Endocrinology. 1987 Jan;120(1):299-304. doi: 10.1210/endo-120-1-299.
The possible role of atrial natriuretic factor (ANF) in the regulation of adrenal sensitivity to angiotensin II (AII) was investigated in vivo and in vitro by analyzing the characteristics of the inhibitory effect of ANF on aldosterone production stimulated by AII and other stimuli. In isolated adrenal glomerulosa cells, ANF caused a dose-dependent inhibition of basal and stimulated aldosterone production by submaximal concentrations of ACTH, AII, and potassium, with an ED50 of about 1 nM for ANF and complete inhibition with 10 nM ANF. ANF increased the ED50 for ACTH from 14.6 +/- 3.2 to 376 +/- 104 pM with no significant decrease in the maximum aldosterone response. In contrast, ANF inhibited the aldosterone responses to all doses of AII, decreasing maximal aldosterone production by 75%, with a small increase in the ED50 for AII. In conscious rats, ANF infusion (100 ng/min) markedly decreased the plasma aldosterone response to AII infusion (5-10 ng/min). With higher AII doses (50 and 100 ng/min), which increased plasma corticosterone (and presumably ACTH secretion), the inhibitory effect of ANF was less marked. When the rise in ACTH secretion was prevented by dexamethasone treatment, ANF decreased the aldosterone response to 100 ng/min AII by 85%. Similarly, ANF had a minor although significant inhibitory effect on the primary ACTH-mediated increases in plasma aldosterone after stress by immobilization for 15 min. The data demonstrate a prominent inhibitory effect of ANF on AII-stimulated aldosterone production in vivo and in vitro. Since plasma ANF levels are increased during atrial distension, these observations support a regulatory role of ANF in the control of the adrenal sensitivity to AII during alterations of extracellular volume.
通过分析心房利钠因子（ANF）对血管紧张素II（AII）及其他刺激所诱导的醛固酮生成的抑制作用特点，在体内和体外研究了ANF在调节肾上腺对AII敏感性方面可能发挥的作用。在分离的肾上腺球状带细胞中，ANF对促肾上腺皮质激素（ACTH）、AII和钾离子亚最大浓度刺激下的基础及刺激型醛固酮生成产生剂量依赖性抑制，ANF的半数有效剂量（ED50）约为1 nM，10 nM ANF可完全抑制。ANF使ACTH的ED50从14.6±3.2 pM增至376±104 pM，醛固酮最大反应无显著降低。相比之下，ANF抑制对所有剂量AII的醛固酮反应，使醛固酮最大生成量降低75%，AII的ED50略有增加。在清醒大鼠中，输注ANF（100 ng/min）显著降低血浆醛固酮对输注AII（5 - 10 ng/min）的反应。使用更高剂量的AII（50和100 ng/min）时，血浆皮质酮升高（推测促肾上腺皮质激素分泌增加），ANF的抑制作用减弱。当通过地塞米松处理防止促肾上腺皮质激素分泌增加时，ANF使醛固酮对100 ng/min AII的反应降低85%。同样，ANF对固定15分钟应激后原发性促肾上腺皮质激素介导的血浆醛固酮增加有轻微但显著的抑制作用。数据表明，ANF在体内和体外对AII刺激的醛固酮生成有显著抑制作用。由于心房扩张时血浆ANF水平升高，这些观察结果支持ANF在细胞外液量改变期间控制肾上腺对AII敏感性方面的调节作用。
