三磷酸腺苷（ATP）可促进视杆细胞环磷酸鸟苷磷酸二酯酶的激活与失活。完整视杆细胞外段的动态光散射。

ATP can promote activation and deactivation of the rod cGMP-phosphodiesterase. Kinetic light scattering on intact rod outer segments.

作者信息

Kamps K M, Hofmann K P

出版信息

FEBS Lett. 1986 Nov 24;208(2):241-7. doi: 10.1016/0014-5793(86)81025-0.

Abstract

The AT (amplified transient) signal is a flash-induced increase of the near-infrared light scattering from isolated bovine rod outer segments and is interpreted as a monitor of cGMP-phosphodiesterase activation [(1985) FEBS Lett. 188, 15-20]. We have investigated the effects of ATP and cyclic GMP on this signal. It has been found that ATP enhances the AT signal, the relative effect being the largest for low photoexcitation (approximately 1 rhodopsin per disc membrane). At a high rhodopsin turnover, which saturates the AT amplitude, the effect of ATP is to accelerate the rise of the signal. ATP can also accelerate the falling phase of the signal. This deactivating effect depends on the simultaneous presence of cyclic GMP. The results indicate that ATP acts on the phosphodiesterase activation cycle, promoting activation as well as deactivation, dependent on cGMP as a cofactor.

摘要

AT（放大瞬态）信号是由闪光诱导的、来自分离的牛视杆细胞外段的近红外光散射增加，被解释为cGMP-磷酸二酯酶激活的监测指标[（1985年）《欧洲生物化学学会联合会快报》188, 15 - 20]。我们研究了ATP和环鸟苷酸（cGMP）对该信号的影响。已发现ATP增强AT信号，对于低光激发（每个盘膜约1个视紫红质），相对效应最大。在视紫红质周转高、使AT振幅饱和时，ATP的作用是加速信号上升。ATP还能加速信号的下降阶段。这种失活效应取决于环鸟苷酸同时存在。结果表明，ATP作用于磷酸二酯酶激活循环，促进激活以及失活，依赖环鸟苷酸作为辅因子。