Feit C, Sen G, Bartal A H, Hirshaut Y, Anderson S L, Millet S K, Rubin B Y
Exp Cell Biol. 1986;54(4):212-9. doi: 10.1159/000163357.
An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-gamma) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-gamma, the ability of HuIFN-gamma to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-gamma to induce the 2'-5' oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE.
在细胞融合初始阶段，使用添加胰岛素的HAT培养基（HIAT）产生了一种杂交瘤克隆，其分泌对人γ干扰素（HuIFN-γ）具有特异性的IgG1单克隆抗体（GIF-1）。该抗体能够中和HuIFN-γ的抗病毒活性、HuIFN-γ抑制RD-114细胞中逆转录病毒复制的能力，以及HuIFN-γ诱导RD-114和HeLa细胞中2'-5'寡腺苷酸（A）合成酶的能力。当对含有GIF-1的免疫亲和柱洗脱液进行SDS-PAGE时，得到了两条分子量分别为20和25kd的蛋白带。
